1. School of Chemistry and Chemical Engineering, Henan University of Science and Technology, Luoyang 471000, China; 2. Ocean College, Zhejiang University, Zhoushan 316021, China
Abstract：A biosurfactant (BS)-producing strain YY-1, which was isolated from high-salt oily wastewater, was identified as Shewanella based on morphological, physiological, biochemical characteristics and 16S rRNA gene sequences analysis. The critical micelle concentration of the BS secreted by Shewanella sp. YY-1 was 700mg/L, the surface tension was 30.76mN/m, and the BS showed good chemical stability over a wide range of salinity (10~100g/L), temperature (25~100℃) and pH (2~10). The extracted BS were identified as phospholipids according to thin-layer chromatography, fourier infrared spectroscopy and gas chromatography- mass spectrometry. The results of bioremediation of crude oil wastewater with strain Shewanella sp. YY-1 showed favourable degradation performance. The removal efficiency of short-chain alkanes (C8~C10), medium-chain alkanes (C11~C20), and long-chain alkanes (C21~C44) were 74.70%, 96.68%, and 99.42%, respectively. This study could provide excellent strain resource and theoretical basis for the bioremediation of crude oil wastewater with BS-producing bacteria.
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