铜绿微囊藻对自养氨氧化细菌的抑制作用

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摘要 Using Microcystis aeruginosa FACHB-1027 and autotrophic ammonia-oxidizing bacteria (AOB) Nitrosomonas eutropha CZ-4 and Nitrosomonas nitrosa WH-1 as the research material,this study explored the inhibitory effect of M. aeruginosa on AOB.Also,the molecular weight range of the inhibitory substances was determined by dialysis bag tests and the inhibitory substances composition was analyzed by GC-MS.The results showed that the inhibition efficiency of M. aeruginosa on the ammonia oxidation activities of the two AOBs were 90.7% and 89.4%,respectively,indicating that M. aeruginosa can significantly inhibit the ammonia oxidation activity of AOB in a non-ammonium nitrogen competitive manner.After the M. aeruginosa cultures were treated with a dialysis bag with a molecular weight cut-off of 200 Da,the permeate inhibited the ammonia oxidation activity of AOB by 86.9%. The half inhibitory concentration (IC50) of 5,5-2 methyloxazolidine-2,4-dione in the permeate to the ammonia oxidation activity of AOB was 0.4‰~0.8‰,indicating that the M. aeruginosa can secrete small molecular to inhibit the ammonia oxidation activity of the AOB.

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	冯倩倩
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关键词 ：水华, 铜绿微囊藻, 氨氧化细菌, 抑制, 5, 5-2甲基恶唑烷2, 4二酮

Abstract：Using Microcystis aeruginosa FACHB-1027 and autotrophic ammonia-oxidizing bacteria (AOB) Nitrosomonas eutropha CZ-4 and Nitrosomonas nitrosa WH-1 as the research material,this study explored the inhibitory effect of M. aeruginosa on AOB.Also,the molecular weight range of the inhibitory substances was determined by dialysis bag tests and the inhibitory substances composition was analyzed by GC-MS.The results showed that the inhibition efficiency of M. aeruginosa on the ammonia oxidation activities of the two AOBs were 90.7% and 89.4%,respectively,indicating that M. aeruginosa can significantly inhibit the ammonia oxidation activity of AOB in a non-ammonium nitrogen competitive manner.After the M. aeruginosa cultures were treated with a dialysis bag with a molecular weight cut-off of 200 Da,the permeate inhibited the ammonia oxidation activity of AOB by 86.9%. The half inhibitory concentration (IC50) of 5,5-2 methyloxazolidine-2,4-dione in the permeate to the ammonia oxidation activity of AOB was 0.4‰~0.8‰,indicating that the M. aeruginosa can secrete small molecular to inhibit the ammonia oxidation activity of the AOB.

Key words： bloom microcystis aeruginosa ammonia oxidizing bacteria inhibition 5,5-2 methyloxazolidine 2,4dione

收稿日期: 2024-04-26

PACS:

X172

基金资助:国家级大学生创新创业训练计划项目(202310500020);河湖健康智慧感知与生态修复教育部重点实验室开放基金项目(HGKFYBP06)

通讯作者: 程凯,教授,chengkaicn@163.com E-mail: chengkaicn@163.com

作者简介: 冯倩倩(1997-),女,安徽安庆人,湖北工业大学硕士研究生,主要研究方向为水体微生物生态.发表论文1篇.2298783587@qq.com.

引用本文:

冯倩倩, 夏茹婷, 梅洪, 程凯. 铜绿微囊藻对自养氨氧化细菌的抑制作用[J]. 中国环境科学, 2024, 44(11): 6332-6340. FENG Qian-qian, XIA Ru-ting, MEI Hong, CHENG Kai. Inhibition effect of Microcystis aeruginosa on autotrophic ammonia-oxidizing bacteria. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(11): 6332-6340.

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