Soil ecotoxicological effects of toluene, ethylbenzene and xylene (TEX) were investigated using earthworm (Eisenia fetida) and wheat (Triticum aestivum L.). The results showed that there were significantly positive correlations between the lethal or inhibitory effects on Eisenia fetida and Triticum aestivum L. and the tested concentration of the three pollutants. The 24h LC50 values of TEX toxic to Eisenia fetida were 583.6, 346.8 and 192.4mg/kg, and the 48h LC50 values were 454.3, 167.1 and 127.2mg/kg, respectively. The IC10 values based on the toxicity of TEX to shoot elongation of Triticum aestivum L. were 342.2, 195.4 and 45.9mg/kg, and the IC10 values based on the toxicity of TEX to root elongation were 206.7, 134.5 and 26.3mg/kg, respectively. Although both the root and shoot elongation of wheat could indicate the pollution extent of TEX in soil, root elongation was more sensitive than shoot elongation under the pressure of the three pollutants, and there was a significantly positive correlation between these two indexes.