Response characteristics of a single-stage autotrophic nitrogen removal system to graphene-based materials

GUO Li-sha; SONG Bing-hao; ZHU Lei; CHEN Jing; WU Sha; CHEN Hong; ZHANG Jing

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China Environmental Science ›› 2026, Vol. 46 ›› Issue (1) : 178-187.

Water Pollution Control

Response characteristics of a single-stage autotrophic nitrogen removal system to graphene-based materials

GUO Li-sha¹, SONG Bing-hao^1,2, ZHU Lei³, CHEN Jing^1,4, WU Sha^1,4, CHEN Hong^1,4, ZHANG Jing¹

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Abstract

Graphene oxide-chitosan (GO-CS) composite materials were prepared using the hydrothermal method, and a single-stage autotrophic nitrogen removal reactor was constructed for continuous experiment. Batch experiments were conducted to evaluate its concentration-response characteristics, and molecular biology method was applied to elucidate the succession patterns of microbial communities. The effect of GO-CS addition on the performance of single-stage autotrophic nitrogen removal was systematically investigated. The results showed that 20mg/L GO-CS promoted anaerobic ammonium oxidation by facilitating electron transfer; at a concentration of 50mg/L, the activity of AnAOB was inhibited which might be there were other reactions which competing for the use of GO-CS as an electron transfer; and after long-term exposure to 100mg/L GO-CS, AnAOB achieved adaptive recovery by increasing the secretion of extracellular polymeric substances (EPS) and regulating functional genes.

Key words

chitosan / graphene oxide / anaerobic ammonium oxidation (anammox) / microorganisms

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GUO Li-sha, SONG Bing-hao, ZHU Lei, CHEN Jing, WU Sha, CHEN Hong, ZHANG Jing. Response characteristics of a single-stage autotrophic nitrogen removal system to graphene-based materials[J]. China Environmental Science. 2026, 46(1): 178-187

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