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| Microbial source tracking of the pollution in pond-drinking water in a country of Guangdong |
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Abstract Pollution sources of the typical rural pond-drinking water were studied in a country of Guangdong using molecular typing methods such as pulsed-field gel electrophoresis (PFGE), ERIC-PCR and BOX-PCR. Escherichia coli was the indicating microorganism in microbial source tracking (MST). The fingerprints of E. coli from different samples were analyzed using Quantity One. It was showed that the superficial layer well had the same genotyping E. coli with the pond water beside it. Therefore, they might have the same pollution carrier. Or the pond resulted in the pollution of superficial layer well. The ability in discriminating E. coli strains was different, such as PFGE>BOX-PCR>ERIC-PCR. BOX-PCR was the most suitable molecular typing method for microbial source tracking. In view of limitation of the culture-dependent methods, we should pay more attention to the combination with culture-independent methods in the future MST.
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Received: 07 May 2010
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