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| Isolation and identification of an alpha-pinene-degrading bacterium and its degradation characteristics |
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Abstract An aerobic bacterium (strain PT) capable of degrading α-pinene was isolated from the biofilm of a biotrickling filter. Based on its morphology, physiological-biochemical characteristics, 16S rRNA gene sequence analysis and Biolog analysis, the strain PT was identified as Pseudomonas fluorescens. The results of response surface methodology (RSM) indicated that the initial conditions of NaCl concentration 0.00%, pH7.31 and temperature 25.5°C were optimal for its growth with the maximum biodegradation rate. Haldane’s kinetics model could be fit to the kinetics date well and the maximum specific degradation rate was 0.0364h-1. The strain PT could biodegrade several industrial contaminants which were characterized with simple or similar structures to that of α-pinene. GC/MS analysis showed that α-pinene was converted to some simple organic compounds (limonene, perillic acid etc.) by the strain PT, which was finally mineralized to CO2, H2O and synthesized to cell biomass. Analysis of carbon balance indicated that the rates for the mineralization and the biomass synthesis were about 64.83% and 30.37%, respectively.
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Received: 31 August 2010
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