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| Application of two assays for damage assessment of damage caused by drinking water disinfection by-products in HepG2 cells |
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Abstract Cytotoxicity and genotoxicity of drinking water disinfection by-products (DBPs) in HepG2 cells was to evaluate using crystal violet-colorimetric assay and the cytokinesis-block micronucleus test, and the feasibility of introducing these assays into evaluation system for drinking water safty was explored. HepG2 cells were treated with five DBPs which were chosen based on their genotoxicity and carcinogenicity. The descending rank order in cytotoxicity was 3- chloro -4 - (dichloromethyl) -5 hydroxy -2 (5 H) – furanone (MX) > dichloroacetonitrile (DCAN) > dibromoacetic acid (DBA) > dichloroacetic acid ( DCA ) > trichloroacetic acid (TCA), with a value of cytotoxic potency (% C ? values) ranged from 89.74 (MX) to 9335.38 (TCA) μmol/L. Micronuclei occurring frequency significantly increased in HepG2 cells treated with five DBPs. The rank order of their genotoxicity among the five DBPs was the same as that of their cytotoxicity. The results indicated that these two assays are feasible for assessing the drinking water safety.
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Received: 14 July 2009
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