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| Characterization of SMP in the effluent of thermophilic anaerobic digestion process treating synthetic glucose wastewater |
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Abstract A thermophilic anaerobic sequenced batch reactor was applied to treat synthetic glucose wastewater in order to investigate the characteristics of soluble microbial product (SMP) in the effluent of thermophilic anaerobic digestion process. The dissolved organic matter (DOM) in the effluent was fractionated using size exclusion chromatography and characterized by dissolved organic carbon content (DOC), UV254 absorbance and fluorescence excitation-emission matrix. The SMP accounted for 90.8% of the DOM in the effluent. The distribution ratios of SMP in high-molecular weight (MW) (10k~100kDa), medium-MW (1k~10kDa) and low-MW (<1kDa) DOM fractions were 46.8%, 45.4%, 7.8%, respectively. The DOM fraction with high MW showed higher ultraviolet absorptivity than that with low and medium MW. The SMP was characterized by relatively high content of tyrosine-like substances and low content of fulvic acid-like and humic acid-like substances. Low-MW fraction mainly contained tyrosine-like aromatic substances, whereas all fulvic acid-like and humic acid-like substances distributed in the medium-MW fraction, and tryptophan-like protein and soluble microbial by-product-like substance extensively distributed in different MW range fractions. There was no significant difference between the fluorescence profiles of high and medium MW fractions, except a little change in the structures of tryptophan-like aromatic protein and soluble microbial by-product-like substances.
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Received: 06 August 2009
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