Abstract Acute toxicities of heavy metals (Hg2+, Cu2+, Zn2+, Cr6+, Cd2+, Pb2+ and Co2+), organic compounds (2-CP, 2,4-DCP, 2-NP, 4-NP, tetracycline and SDBS)and oil wastewater were determined with Bacillus subtilis microbial biosensor. When Bacillus subtilis was in logarithmic and stationary phase, the microbial biosensor had good performance for acute toxicity analysis, the EC50 of Hg2+, Cu2+, Zn2+, Cr6+, Cd2+, Pb2+, 2-CP, 2,4-DCP, 2-NP, 4-NP, tetracycline and SDBS to Bacillus subtilis were 47.3, 10.9, 14.0, 2.6, 0.8, 100.1, 559.6, 450.8, 588.5, 487.0, 121.3 and 558.9 mg/L, respectively, the EC30 of Co2+ was 56.6mg/L. The microbial biosensor could reflect the actual toxicity of oil field wastewater.
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