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| Assessment on genotoxicity and health risks of microcystin in drinking water sources |
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Abstract To assess the genotoxicity of microcystin (MC) in drinking water source for bacteria, carp and human, high health risk of MC-LR was observed, based on concentrations of organic pollutants in 101 drinking water sources of Zhejiang province. Surface water of 2 typical drinking water source A and B were sampled, which were relatively seriously polluted by MC-LR. The diluted microcystin of MC-LR was added to the water samples, while MC-LR-containing purified water was prepared. The HLB resin was used to absorb and concentrate the microcystin in source water samples. The bacteria, human peripheral blood lymphocytes and ornamental carp erythrocyte were treated by purified water, drinking water source A and B, concentrated water samples, and MC-LR-containing purified water, respectively. The mutation of bacterial DNA was assessed by Ames test. The DNA damage of human peripheral blood lymphocytes was tested by comet assay. Micronucleus of ornamental carp erythrocyte was measured by micronucleus test. Compared with purified water, drinking water source A and B, concentrated samples, and MC-LR-containing purified water resulted in DNA damage of human peripheral blood lymphocytes (P<0.01). With the concentration of MC-LR increasing, the level of DNA damage was growing up. Likewise, concentrated water samples and MC-LR-containing purified water were able to induce higher frequency of micronucleus in carp erythrocyte. However, DNA mutation induced by concentrated water samples, drinking water source A and B, and MC-LR-containing purified water were not observed. It is practical to simulate the microcystin-releasing with MC-LR dissolving in drinking water source. Microcystin in drinking water source could induce micronucleus in carp erythrocyte and DNA damage in human lymphocytes. It indicated that MC-LR may have the potential adverse effects on human health.
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Received: 27 August 2009
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ZHAN Xiao-Jing, XIANG Lei, LI Yan-Wen, MO Ce-Hui, DENG Zhe-Shen, HUANG Bin-Hui, WEN Hong-Fei, CAI Quan-Ying, ZHAO Hai-Ming. Investigation and risk evaluation of microcystins in agricultural soils[J]. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(7): 2129-2136. |
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