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| Effect of di-n-butyl phthalate (DBP) on the microbial diversity in shallow aquifer sediment |
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Abstract Impact of di-n-butyl phthalate (DBP), an endocrine-disrupting chemical, on microbial community diversities and activities in shallow aquifer sediments in Jianghan plain were investigated in batch cultures via BIOLOGECO microplates. Sediments were amended with different concentrations of DBP varied from 0 to 796mg/kg and incubated under a condition of hypothermia-hypoxia which was commonly found in subsurface aquifer. Average well colour development (AWCD) was measured with mircosation biolog periodically. It was observed that the microbial populations and activities varied with DBP concentrations. With the variation of DBP concentrations no obvious difference was detected among the richness (R) and Shannon’s index (H), while remarkable differences was found among the Simpson’s indices and McIntosh’s indices of the control and sediments amended with DBP (P<0.05). The microbial activity which might potentially related to DBP degradation was enhanced by DBP with low concentration (<400mg/kg). However, with higher concentrations, (3796mg/kg), DBP grossly inhibited microbial growth and metabolism as manifested by the reduction in microbial count, and destabilized the natural balance of microbial communities.
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Received: 25 September 2009
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