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| Study on production of peroxide hydrogen by Chattonella marina Japan strain |
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Abstract The induced peroxide hydrogen (H2O2) under different stages of C. marina which was treated at different salinity and nutrient conditions was studied. The results showed that high concentration of H2O2 was observed during the logarithmic phase from 4 to 8 d, with the maximum value of 0.97×10-4nmol/cell on the 6th day. Under the N:P ratio of 8:1 and 16:1, CMJP possessed the relative higher growth rate and cell concentration. However, H2O2 concentration was relatively low at those optimal N:P ratios, indicating the production of H2O2 is negative to the growth of C. marina. The lowest concentration of peroxide hydrogen of CMHK was 0.40×10-4nmol/cell with the N:P ratio of 16:1, which was only one third of that with the N:P ratio of 32:1 (1.17 ×10-4). The concentration of peroxide hydrogen per cell was 0.63 ×10-4nmol/cell when N:P ratio was 8:1. The optimal salinities for the growth of CMJP were 20 and 25psu, where cell concentrations were relatively higher. However, cell concentrations were lower at the salinity of 10, 15 and 30psu, showing the growth of CMJP was depressed at relatively low and high salinity. Salinity also influenced the production of peroxide hydrogen. The concentration of peroxide hydrogen per cell was 1.1 ×10-4nmol/cell, the highest value among the experiments, at the salinity of 30 psu.
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Received: 17 February 2011
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