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Comparison of amoA mRNA during inhibition of nitrifying activity by heavy metals |
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Abstract Comparison of amoA mRNA (ammonia mono-oxygenase encoding mRNA) was examined due to inhibition from Cd2+ (1, 5, 10mg/L) and Zn2+(5, 20, 50mg/L) during a short reaction period (6h). The real-time reverse transcription- PCR method was applied to detect profile of amoA mRNA in activated sludge quantificationally. Different performance of mRNA was observed during Cd2+ and Zn2+ inhibition test respectively, the highest inhibitory rate up to 53% at 10mg/L Cd2+ was discovered. The nitrifying inhibition was possibly performed through the impact on transcription of amoA mRNA by some heavy metals.
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Received: 14 January 2010
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[1] |
CHEN Huan-Yu, LIU Jing-Qing, WEI Zong-Yuan, ZHANG Hui-Hui, HE Xiao-Fang, ZHOU Xiao-Yan, QIU Shang-De, HU Bao-Lan, LOU Li-Ping. Radial differences in contaminant distribution in large-diameter pipe scales of main pipelines in drinking water distribution system[J]. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(9): 2706-2712. |
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