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| Isolation, identification and characteristics of a difenoconazole-degrading bacterial strain B2. |
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Abstract A bacteria strain B2 capable of utilizing difenoconazole as sole carbon source was isolated from sludge collected from a pesticide manufacturer which has been producing difenoconazole for many years. It was identified as Ensifer sp. according to its physiological-biochemical properties and the analysis of its 16S rRNA gene sequence (GenBank Accession No. EU303300). Above 85% of 100mg/L difenoconazole was degraded by strain B2 within 24h. The optimal pH and temperature for degradation were 7.0 and 30~35℃ respectively. The degrading rate showed a positive correlation with the amount of initial inoculum size while a negative correlation with the initial concentration of difenoconazole. No degradation occurred if the initial concentration of difenoconazole excessed 400mg/L in mineral salt medium.
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Received: 04 June 2008
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