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| Isolation, identification and characteristics of quinone compounds enhancing dye-decolorizing bacterium. |
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Abstract In the presence of quinone compounds, a strain (designated as JL) with the capacity to decolorize azo dyes was isolated. The strain was identified as Bacillus cereus JL based on morphological, 16S rDNA and 16S-23S spacer region sequence analysis. The optimal conditions for the decolorization of Acid Red 3R by strain JL were: 1g/L glucose, pH 5~7, 30℃, inoculation amount 0.25g/L. Anthraquinone-2-sulfonate (AQS), anthraquinone-2,6-disulfonate (AQDS) and 2-hydroxy-1,4-naphthoquinone (Lawsone) could improve the decolorization rate of Acid Red 3R,and AQS was most effective. Addition of 0.1 mmol/L AQS significantly increased the decolorization rate of 2.0mmol/L Acid Red 3R by strain JL,and the strain could decolorize other six azo dyes. According to the decolorization product of Acid Red 3R identified using HPLC-MS, strain JL could make cleavage for azo bond and AQS only acted as redox mediator in whole process.
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Received: 16 July 2008
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