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| Induction and function of cytochrome P450 in degradation of refractory organic chemicals by Phanerochaete chrysosporium. |
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Abstract The involvement of P450 in degradation of polycyclic aromatic hydrocarbons and chlorophenols by white rot fungus Phanerochaete chrysosporium was investigated. The significant induction of P450s by naphthalene, phenanthrene, 2,4-dichlorophenol and pentachlorophenol were observed. The specific concentrations of microsomal P450s were up to 37~137 pmol/mg in the induced cells. The content of phenanthrene-induced P450 from nutrient-limited culture was 5.7 times higher than that from nutrient-sufficient culture. Piperonyl butoxide (PB), a P450 inhibitor, markedly inhibited the degradation of naphthalene, phenanthrene and pyrene in both nutrient-limited and nutrient-rich culture medium. Moreover, in nutrient-limited culture medium, PB inhibited 2,4-diclorophenol degradation but had no effect on degradation of 2,4,6-triclorophenol and pentachlorophenol. In nutrient-rich culture medium, in contrast, PB inhibited 2,4,6-triclorophenol degradation but did not affect 2,4-diclorophenol degradation. The degradation of pentachlorophenol was even elevated with the presence of PB in nutrient-rich cultures.
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Received: 16 September 2008
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ZHANG Li, ZHANG Yuan, QI Shi-Hua, LI Hui, XING Xin-Li, ZHANG Ze-Zhou, ZHENG Huang, SUN Yan, MIN Yang. Characteristics of atmospheric PM2.5 and the variation of PAHs in PM2.5 during spring in Hongshan district, Wuhan[J]. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(8): 2319-2325. |
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