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Isolation of an efficient aniline-degrading bacterial strain and its application for accidents. |
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Abstract A bacterial strain AN-P1 capable of efficient aniline-degrading was isolated from activated sludge of a sewage treatment plant. It was identified as Rhodococcus sp.. Enzymatic analysis showed that aniline was degraded through meta-cleavage pathway. The optimal conditions for cell growth and aniline degradation were pH 6, 30℃ with an inoculation amount of 0.30‰ and an aniline concentration of 2000 mg/L. PCR-DGGE profile showed that AN-P1 could efficiently protect the microbial community of the activated sludge system against sudden aniline pollution. It took only 10, 20, 32h, respectively,for the treatment of wastewater containing 500, 1000, 2000mg/L aniline to meet the first grade of national integrated wastewater discharge standard. With a combination of AN-P1 and SBR system,it took only 36h for the emergency treatment of wastewater containing 2000 mg/L aniline to meet the discharge standard.
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Received: 18 March 2009
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