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| Effects of C /N ratio on physiological biochemical characteristics of anaerobic granular sludge |
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Abstract Eeffects of initial influent carbon-nitrogen ratio on fermentative types and methanogenic activity of anaerobic granular sludge were studied using the group of experiment which adding molybdate to restrain the activity of methanogens to study the effect of C/N ratio on anaerobic granular sludge during the acidification period, when the initial C/N ratio was 12,56,156,the type of fermentation was butyric acid type fermentation and when the initial C/N ratio was 200, forming ethanol type fermentation. Different C/N ratio had influence on EPS of anaerobic granular sludge during acidification period. With the C/N ratio decreased, the total amount of EPS, extracellular polysaccharide and extracellular proteins had shown a decreasing trend after the first increase, reaching maximum when C/N ratio was 56. Using the group of experiment which did not add molybdate to study the effect of methanogenic activity of anaerobic granular sludge, it had the highest COD removal efficiency and methanogenic activity when C/N ratio was 200. With the C/N ratio decreased, the total amount of EPS had shown a increasing trend after the first decrease .The total amount of EPS reached maximum when C/N ratio was 200 and the extracellular proteins reached maximum when C/N ratio was 156. The analysis of functional group of surface of anaerobic granular sludge with Fourier transform infrared dspectrometry showed when C/N ratio was 200, there was 1350~1260 band peak. The other three C/N ratio had no 1350~1260 band peaks.
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Received: 26 May 2011
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