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| A novel detection method of microcystin of lake water by surface plasmon resonance technique |
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Abstract Amount of Microcystin-LR in water was rapidly and quantitatively detected by SPR (surface plasmon resonance) technique. After the MC-LR being immobilized on the surface of the chip, the stability of the chip was tested, and the standard curve was constructed by the principle of competitive immune inhibition. The results showed that the relative standard deviation of 50 cycles was 0.62%, which represented the stability of the immobilized chip. And the limited concentration of detection is arrived at 0.25ng/ml.. For its stability and sensitivity, the SPR detection method would be applied to assay of MC-LR in the water.
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Received: 03 November 2011
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ZHAN Xiao-Jing, XIANG Lei, LI Yan-Wen, MO Ce-Hui, DENG Zhe-Shen, HUANG Bin-Hui, WEN Hong-Fei, CAI Quan-Ying, ZHAO Hai-Ming. Investigation and risk evaluation of microcystins in agricultural soils[J]. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(7): 2129-2136. |
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