|
|
Purification progress of laccase by reversed micelle system |
|
|
Abstract Backward extraction of laccase by an innovative method, biosurfactant-based reversed micellar extraction (RME) was studied. In this reversed micelle system, rhamnolipid (RL), a kind of anionic biosurfactant, was employed and isooctane was used as solvent. Four different salts type (NaCl, NaBr, KCl and KBr) with different concentration and four different co-solvent type(n-butanol, n-hexanol, n-heptanol and n-octanol) with different ratio were added respectively in the RME. The effects of ethanol and temperature on backward extraction were studied. The experimental results showed that the impact of KCl (0.25mol/L) on backward extraction approximately equal to KBr (0.20mol/L). Optimum co-solvent was n-hexanol or n-heptanol both with the ratio of 50%. Ethanol was added in backward extraction process and the appropriate volume was 9%. The optimum temperature during backward was 35℃. The best activity recovery and purification fold were 90% and 4.7. The analysis results of SDS-PAGE further proved that laccase was successfully purified. The research indicated RL-RME has huge potential in extraction industry.
|
Received: 30 August 2012
|
|
|
|
|
|
|