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Construction of whole cell biosensor Tetrahymena B2086-LUC containing luciferase and its response to heavy metal ion. |
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Abstract :To obtain new whole cell biosensor which can be used for rapidly detecting metal ion pollution in the environment, Tetrahymena thermophilia was used in this study. Luciferase (LUC) gene was recombinated into the vector pBX containing Tetrahymenna Metallothioneins gene MTT1 promoter and tubulin terminator. Plasmid pBX–LUC was constructed and transformed into Tetrahymena strain by particle bombardment. Then, the LUC gene replaced the Tetrahymena macronuclear MTT1 gene by homologous recombination under increasing paromomycin. The B2086-LUC strain was obtained. The B2086-LUC strain is sensitively responsed to heavy metals cadmium and mercury ion (5 ~ 10 ng/mL) and less sensitive for copper and zinc ion (0.5~1 mg/mL). The results showed genetically engineered whole cell biosensor Tetrahymena B2086-LUC strain can be used for rapidly detecting cadmium and mercury pollution in environment.
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Received: 08 November 2012
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