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| Geosmin production characteristics by Anabaena sp. in nitrogen-limited and phosphorus-limited conditions |
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Abstract Characteristics of Geosmin produced by Anabaena sp. under phosphorus-limited and nitrogen-limited conditions were investigated in batch cultivation and determined using SPME-GC Mass. Anabaena sp. grew well and the growth rate was not influenced by nitrogen limitation. However, its growth was restrained by phosphorus limitation. Heterocyst was produced and its percentage account for 3.5%~4.4% of total cells under nitrogen-limited condition, whereas heterocyst was not produced under phosphorus-limited condition. The geosmin content in cell declined quickly in the first 20d and then kept almost constant. The geosmin content in the late phase was 3.7×10-5ng/cell under nitrogen-limited condition and was a little higher under phosphorus-limited condition (3.2×10-5ng/cell). Chl a concentration under the nitrogen-limited condition was much higher than that under phosphorus-limited condition. The ratio of geosmin content to Chl a content (geosmin/Chl a) under the phosphorus-limited condition (2.3×10-2) was higher than that under nitrogen-limited condition (1.5×10-2).
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Received: 13 August 2013
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