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| Study on sensitivity and specificity of the Bacteroidales biomarkers for microbial source tracking in the Pearl River Delta region |
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Abstract A total of 33 fecal samples of human and different animal species were collected in the Pearl River Delta region. Genomic DNA were then efficiently extracted and amplified by using 14 specific Bacteroidales primers that have been reported as tracking markers of different sources. Primers with high specificity and sensitivity were then selected and used for polluted water samples analysis. The results show that the purity and extract efficiency of the genome DNA meet the requirement of the following experiments. 85% of the 33 fecal samples analyzed were positive when using Bacteroidales universal primer 2#(Bac32F/Bac708R), and most of the mammalian animals and chicken fecal samples were detected as positive samples when use this primer. Human-specific marker 3#(HF134F/Bac708R) and 4#(HF183F/Bac708R), ruminant-specific marker 8#(CF128F /Bac708R)and porcine-specific marker 10#(PF163F/Bac708R)showed high sensitivity and specificity in the Pearl River Delta region. Polluted water samples analysis results were the same as the actual type of pollution, implying Bacteroidales universal primer 2#, human-specific primer 3# and 4# and porcine-specific primer 10# could be used as pollution tracking markers in the Pearl River Delta region.
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Received: 02 December 2013
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