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| Fermentation and kinetics characteristics of bioflocculant production using swine wastes as raw material |
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Abstract Swine wastewater was used as raw material to produce bioflocculant. Concentration of dissolved oxygen (DO), COD, ammonium, total phosphorus (TP) and solution pH values in the fermentation process were examined. Growth and producing kinetics models of the bacteria were described by Logistic and Luedeking-Piret equations. During the logarithm phase (0~60h), especially in 12~36h, cells grew rapidly. Cell dry weight, cell density OD600, and the number of colonies increased from 0.09g/L, 0.26 to 1.3×107cfu to 0.76g/L, 0.58 and 3.5×107cfu, respectively. The number of colonies reached its maximum value of 5×107cfu at 66h. Protein, the main active ingredient of the metabolite, was produced in parallel with cell growth. Phosphorus in the swine wastewater was essential for adjusting pH values during the flocculation process, and its content was adequate. Furthermore, the cell growth and bioflocculant production simulated very well with both the Logistic and Luedeking-Piret equations.
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Received: 10 February 2014
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