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| Oxidative damage induced by cypermethrin in the kidney tissue of Kunming mice |
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Abstract Kunming mice were randomly grouped into six groups and treated with orally administered drugsona daily base for a week. The groups included one solvent control group, three cypermethrin groups, one high dose cypermethrin plus vitamin E protection group and one vitamin E group. The exposure doses of cypermethrin groups were 10, 20 and 40mg/kg respectively. Some kidney tissues were then made into homogenates for the measurement of reactive oxygen species (ROS), glutathione (GSH), 8-hydroxy-2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) contents. Meanwhile, DNA-protein crosslink (DPC) coefficients were detected from brain cell suspension.The kidney contents of ROS, MDA, 8-OHdG and DPC coefficients increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. In the exposure group with the dose of 20mg/kg, MDA contents and DPC coefficients were significantly higher than those of the control group (P<0.05); ROS (F=3.7044), GSH (F=3.4908), MDA (F=3.5851), 8-OhdG (F=11.7934) and DPC (F=6.9165) levels were significantly different in levels of each biomarker between 40mg/kg group and control group (P<0.05,P<0.01). Furthermore, the high-dosaged (20and 40mg/kg) group had increased glomerulus cells, proliferation and hypertrophy of glomerulus, hydrops of renal tubular epithelial cell, and narrow lumen. Administration of vitamin E (VE) to cypermethrin-treated mice reflected that ROS, MDA, 8-OHdG and DPC increased whereas GSH decreased (P<0.05,P<0.01). Cyermethrin at certain doses (320mg/kg) could induce oxidative stress in mice kidney, whereas vitamin E had antioxidant effects.
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Received: 31 December 2013
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