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| Internally LED-illuminated flat plate photobioreactor for microalgae cultivation-carbon-fixation and production of lipid in Chlorella vulgaris cultured in photobioreactor. |
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Abstract In order to improve the utilization efficiency of light by microalgae within a photobioreactor (PBR), an internally LED-illuminated flat plate PBR with volume of 3L was constructed and used for Chlorophyta (Chlorella vulgaris) in this study. Effects of the concentration of inlet CO2 on the biomass production (BP), fixation efficiency of carbon ( ) and the production of lipid (LP) were evaluated. Biomass of microalgae in each treatment with concentration of CO2 ranging from 1% to 10% was higher than that of control with air (0.04% CO2) aeration in the cultures after 10-days growth. High levels of BP [0.258 and 0.263g/(L?d)], [1.18 and 1.00gCO2/(L?d)] and average FCO2 [0.57 and 0.62gCO2/(L?d)] during the exponential growth stage were obtained under the concentration of CO2 at 1% and 2.5%, respectively. The cell density and biomass of microalgae were inhibited by the higher concentrations of CO2 (5% and 10%) during the initial growth stage because of acidification in cultures. Significant effects of CO2 concentrations on the production of lipid (17.81% ~ 23.13%) were not found here. The highest productivity of lipid [60.71mg/(L?d)] was obtained under the concentration of CO2 at 2.5%. The present results suggested that the PBR used here was useful to improve CO2 biofixation and the production of lipid of microalgae.
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Received: 18 September 2014
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