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| Diversity of actinomycetes at the saline-alkali soils in downstream area of Shiyang River of Hexi Corridor——Illustrated by the case of Minqin County |
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Abstract In order to understand the population structure and diversity of actinomycetes at saline-alkali soils in Shiyang River area of Hexi Corridor, the total DNA was extracted from three types soils (primary saline-alkali soil, secondary saline-alkali soil and farmland soil). Meanwhile, actinomycetes 16S rRNA gene clone libraries were constructed by amplified total DNA products with actinomycetes specific primers, then the positive clone products were digested by HaeⅢ and HhaⅠ, and the clones with different type of electrophoretic bands (Amplifed Ribosomal DNA Restriction Analysis, ARDRA) were sequenced, furthermore, the phylogenetic trees were built and diversity index was analyzed. The results demonstrated that the 90positive clones from the primary saline-alkali soil were attributed to 20 OTUs, belonged to Micrococcineae, Propionibacterineae, Corynebacterineae, Frankineae, Pseudonocardineae, Actinomycetales and unknown groups. The 98 positive clones from the secondary saline-alkali soil were attributed to 32 OTUs, belonged to Micrococcineae, Propionibacterineae, Corynebacterineae, Frankineae, Pseudonocardineae, Actinomycetales and unknown groups. The 98 positive clones from farmland soil were attributed to 10 OTUs, belonged to Micrococcineae, Propionibacterineae, Corynebacterineae, Frankineae, Pseudonocardineae, Actinomycetales and unknown groups. Micrococcineae was the dominant population in all type soils. The diversity index and Rarefaction curves analysis showed that actinomycetes diversity in the three different types soils was in the order secondary saline-alkali soil > primary saline-alkali soil > farmland soil.
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Received: 31 October 2014
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