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Abstract On the basis of microcalorimetric technology, we investigated the effect of glyphosate on metabolic activity of two homologous gene engineering bacteria of Pseudomonas. Glyphosate can serve as nutrients of these two strains.The half inhibitory concentration of glyphosate for P.stutzeri WM 581 and P.stutzeri WM 567 were 47.47mg/L and 43.26mg/L respectively. Both of strains can degrade glyphosate rapidly, and the half-life was approximate 9~17 hours. And as metabolism nutrients of these two strains, the degradation rate of glyphosate as carbon source (64%) is higher than as phosphorus source (43%). Salinity and pH impacted the growth of these two strains remarkably, the relatively optimal condition to degrade glyphosate is: pH = 6, NaCl < 0.5g/L.
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Received: 19 March 2015
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