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Abstract The biotransformation and methylation of arsenic by Shewanella oneidensis MR-1 were studied under laboratory condition with the addition of As (III) and As (V) respectively to the culture medium. The result showed the biotransformation of arsenic by S. oneidensis MR-1 was dependent on microbe tolerance and metabolism. When As (III) was added to the medium, a small quantity of As (V) was generated by S. oneidensis MR-1for detoxification, meanwhile organic arsenic was generated by the action of methyltransferase. When As (V) was added to the medium, monomethylarsine and dimethylarsine were produced step by step through the Challenge mechanism. In addition, weakly acidic environment was more beneficial to arsenic methylation than alkaline condition. When the culture temperature was 30℃, the arsenic methylation rate was higher than others.
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Received: 27 March 2015
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