改良剂对土壤铜镉有效性和微生物群落结构的影响

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Abstract

Concentrations of available Cu and Cd, soil microbial community structure were investigated with one-time application of charcoal, lime, and apatite after four years in a heavy metal contaminated soil. Results showed that incorporation of amendments significantly increased soil pH, and decreased the concentrations of exchangeable acidity and aluminum. Lime and apatite were more effective than charcoal on transforming Cu and Cd from active to inactive fractions. The Biolog analysis showed that AWCD (average well color development) of charcoal (0.61), lime (0.76), and apatite (0.70) were 1.33, 1.65 and 1.52 times higher than that in the untreated soil at 192h. Moreover, the Shannon index, McIntosh index of soil microbes were higher in the amended soils compared with the control, and followed the order of lime > apatite > charcoal. The results indicated amendments treated soils all increased the abilities of the microbes to use carbon sources. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis showed that the amounts of dominant bacterial were significantly increased with the application of amendments. The Shannon index of soil microbes were increased 0.22, 0.39 and 0.24 in charcoal, lime and apatite compared with the control, respectively. Correlation analysis indicated that soil acidity and availability of Cu and Cd may be the main factors affecting the community structure of soil microbes in the soils.

Key words： heavy metal availability biolog PCR-DGGE microbial community structure

Received: 25 May 2015

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X53

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	Articles by authors
	CUI Hong-biao
	FAN Yu-chao
	ZHOU Jing
	SHI Yu
	XU Lei
	GUO Xue-tao
	HU You-biao
	GAO Liang-min

Cite this article:

CUI Hong-biao,FAN Yu-chao,ZHOU Jing等. Availability of soil Cu and Cd and microbial community structure as affected by applications of amendments[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(1): 197-205.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I1/197

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