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| Analysis of microbial community structure in different partial nitrification system |
| HOU Ai-yue, LI Jun, BIAN Wei, WANG Meng, ZHENG Lin-xue, ZHANG Yan-zhuo, ZHAO Xin-yan |
| Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China |
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Abstract In order to study the influences of organic carbon source on the microbial community structure in partial nitrification system, the microbial diversity was investigated in two partial nitrification reactors by the construction of bacterial clone library. The influents of two reactors were artificial wastewater with and without organic carbon source, respectively. The results indicated that Proteobacteria and uncultured bacterium were the dominant bacteria of the two studied systems. The differences existed in the structure of the bacterial community of the two systems. Nonetheless, the dominant bacteria and their proportions were similar to each other in the total clones. In addition, the main denitrifer groups were also similar in the two systems. Due to the different concentrations of organic carbon source, the denitrifer genera and their proportions were distinct. The main denitrifer genera of partial nitrification reactor with inorganic wastewater were Nitrosomonas, Nitrospira and Denitratisoma. The proportion of autotrophic nitrifying bacteria in partial nitrification reactor with inorganic wastewater was higher than that with organic wastewater. But in the reactor with inorganic wastewater, the proportion of autotrophic bacteria was lower than heterotrophic bacteria. The main denitrifer genera of partial nitrification reactor with organic wastewater were some denitrifying bacteria of β-Proteobacteria and Nitrosomonas. Additionally, the proportion of the latter in this reactor was relatively low.
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Received: 10 July 2015
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| [1] |
张功良,李冬,张肖静,等.延时曝气对常温低氨氮SBR亚硝化影响及恢复[J]. 中国环境科学, 2014,34(8):1998-2002.
|
| [2] |
Bartroli A, Carrera J, Perez J. Bioaugmentation as a tool for improving the start-up and stability of a pilot-scale partial nitrification biofilm airlift reactor[J]. Bioresource Technology,2011,102(6):4370-4375.
|
| [3] |
Nittami T, Ootake H, Imai Y, et al. Partial nitrification in a continuous pre-denitrification submerged membrane bioreactor and its nitrifying bacterial activity and community dynamics[J]. Biochemical Engineering Journal, 2011,55(2):101-107.
|
| [4] |
苏东霞,李冬,张肖静,等.曝停时间比对间歇曝气SBR短程硝化的影响[J]. 中国环境科学, 2014,34(5):1152-1158.
|
| [5] |
张亮.高氨氮污泥消化液生物脱氮工艺与优化控制[D]. 哈尔滨:哈尔滨工业大学, 2013.
|
| [6] |
何晓红,杨暖,陶勇,等.高浓度氨氮废水短程硝化及氨氧化菌群分析[J]. 应用与环境生物学报, 2013,19(2):313-317.
|
| [7] |
Beccari M, Passino R, Ramadori R, et al. Kinetics of dissimilatory nitrate and Nitrite reduction in suspended growth culture[J]. Water Pollution Control Federation, 1983,55(1):58-64.
|
| [8] |
Turk O, Mavinic D S. Benefits of using selective inhibition to remove nitrogen from highly nitrogenous wastes[J]. Environmental. Technology. Letters, 1987,8(1):419-426.
|
| [9] |
李凌云,彭永臻,杨庆,等.SBR工艺短程硝化快速启动条件的优化[J]. 中国环境科学, 2009,29(3):312-317.
|
| [10] |
苏东霞,李冬,张肖静,等.不同曝气方式SBR短程硝化试验研究[J]. 中南大学学报(自然科学版), 2014,45(6):2120-2129.
|
| [11] |
赵志瑞,马斌,张树军,等.高氨氮废水与城市生活污水短程硝化系统菌群比较[J]. 环境科学, 2013,34(4):1448-1456.
|
| [12] |
莫旭华,麻威,史荣久,等.氮肥对小麦田土壤nirS型反硝化细菌多样性的影响[J]. 微生物学报, 2009,49(9):1203-1208.
|
| [13] |
Snaidr J, Amann R, Huber I, et al. Phylogenetic analysis and in situ identification of bacteria in activated sludge[J]. Applied & Environmental Microbiology, 1997,63(7):2884-2896.
|
| [14] |
Hill V R, Kahler A M, Jothikumar N, et al. Multi-State evaluation of an ultrafiltration-based procedure for simultaneous recovery of enteric microbes in 100-L tap water samples[J]. Journal of Japan Water Works Association, 2007,76(13):4218-4225.
|
| [15] |
Burrell P, Keller J, Blackall L. Characterisation of the bacterial consortium involved in nit rite oxidation in activated sludge[J]. Water Science & Technology, 1999,39(6):45-52.
|
| [16] |
Lee H, Lee S, Lee J, et al. Molecular characterization of microbial community in nit rate-removing activated sludge[J]. Fems Microbiology Ecology, 2002,41(2):85-94.
|
| [17] |
Tank M, Bryant D A. Nutrient requirements and growth physiology of the photoheterotrophic Acidobacterium, Chloracidobacterium thermophilum[J]. Frontiers in Microbiology, DOI:10.3389/fmicb.2015.00226.
|
| [18] |
李妙婉.一体化脱氮除磷曝气生物滤池处理生活污水的效能及微生物种群结构研究[D]. 济南:济南大学, 2014.
|
| [19] |
黄佩蓓,焦念志,冯洁,等.海洋浮霉状菌多样性与生态学功能研究进展[J]. 微生物学通报, 2014,41(9):1891-1902.
|
| [20] |
郑林雪,李军,胡家玮,等.同步硝化反硝化系统中反硝化细菌多样性研究[J]. 中国环境科学, 2015,35(1):116-121.
|
| [21] |
Dagley S. Catabolism of aromatic compounds by microorganisms[J]. Advances in Microbial Physiology, 1971,6(6):1-46.
|
| [22] |
Caroline S H and Rebecca E P. The β-ketoadipate pathway and the biology of self-identity[J]. Annual Review of Microbiology, 1996,50:553-590.
|
| [23] |
Limpiyakorn T, Kurisu F, Sakamoto Y, et al. Effects of ammonium and nitrite on communities and populations of ammonia-oxidizing bacteria in laboratory-scale continuous-flow reactors[J]. Fems Microbiology Ecology, 2007,60(3):501-512.
|
| [24] |
Lapara T M., Ghosh S. Population dynamics of the ammonia-oxidizing bacteria in a full-scale municipal wastewater treatment facility[J]. Environmental Engineering Science, 2006,23(2):309-319.
|
| [25] |
Limpiyakorn T, Shinohara Y, Kurisu F, et al. Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo[J]. Fems Microbiology Ecology, 2006,54(2):205-217.
|
| [26] |
辛晓东.运行条件对MBR膜污染调控的微生物学研究[D]. 哈尔滨:哈尔滨工业大学, 2013.
|
|
|
|
