<sup>31</sup>P-NMR分析湖泊植物和藻类有机磷方法优化及形态研究

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Abstract

Characterization of organic phosphorus (P_o) from aquatic plants and algae by solution 31P-Nuclear Magnetic Resonance (³¹P-NMR) is important to understand its biogeochemical cycling in lakes. Methods of extraction and selection of parameters for NMR characterization and quantification of P_o critical. Various extractants and radio of sample to extractants were applied for ³¹P-NMR. A mixture of 0.5mol/L NaOH-25m mol/L EDTA with a ratio of sample to extractant of 1:60 was the optimal extraction of P_o from aquatic plants and algae. When the extract was analyzed by use of ³¹P-NMR, the delay time (D1) and scanning time were set to 5s and 15h, respectively, which resulted in approximately 24,000 scans. Based on how Samples were extracted and results of ³¹P-NMR analysis, P in aquatic plants and algae included orthophosphates, orthophosphate monoesters, orthophosphate diesters and pyrophosphate, of which P_o accounted for 34%~53% and 31%~73% respectively. Orthophosphate monoester was the predominant constituent of P_o, which accounted for 92 and 83% of plants and algae, respectively. The proportion of orthophosphate diesters was small in aquatic plants and algae and accounted for only 0~6.65% of the TP. The content of pyrophosphate in algae was approximately 35-fold greater than that in aquatic plants.

Key words： lake aquatic plants algae organic phosphorus ³¹P-NMR

Received: 15 July 2015

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X171.5

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	Articles by authors
	FENG Wei-ying
	ZHU Yuan-rong
	WU Feng-chang
	LIU Sha-sha
	ZHANG Chen

Cite this article:

FENG Wei-ying,ZHU Yuan-rong,WU Feng-chang等. Optimization of extraction and parameters for ³¹P-NMR analysis of organic phosphorus extracted from aquatic plants and algae[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(2): 562-568.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I2/562

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