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| Response of Pseudomonas putida cells to MC-LR stress |
| DENG Ting-jin1, YIN Hua1, YE Jin-shao2, PENG Hui3, LIU Zhi-chen1 |
1. Key Laboratory on Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education College of Environment and Energy, South China University of Technology, Guangzhou 510006, China;
2. College of Environment, Jinan University, Guangzhou 510632, China;
3. Department of Chemistry, Jinan University, Guangzhou 510632, China |
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Abstract The cell integrity and biomass changes of Pseudomonas putida, a MC-LR degrading bacterium, were studied by inoculating 1.0g/L bacterium into systems with different concentrations of MC-LR. The oxidative stress of MC-LR on bacterial cell and the responses of antioxidase were also investigated. The results showed that membrane permeability of P. putida increased under the influence of MC-LR, causing membrane damage, which resulted in the outflow of intracellular substances and the destruction of cell integrity. Also, MC-LR could induce the oxidative stress on the cells of P. putida. With prolonged exposure to MC-LR, reactive oxygen species (ROS) and malondialdehyde (MDA), a product of membrane lipid peroxidation, evidently accumulated in the system, and had obvious dose-effect relationship. Under the effect of MC-LR, the activity of superoxide dismutase (SOD) increased first and then declined, exhibiting an active response to MC-LR of low level. However, after contacting higher concentrations (2.5 mg/L) of MC-LR for 5d, ROS accumulation was so high as to cause damage to the metabolism of cells. As a result, SOD activity was suppressed and cells suffered mass mortality, and the biomass decreased by 50% compared with the control.
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Received: 02 July 2015
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| [1] |
Chen X F, Chuai X M, Yang L Y. Status quo, historical evolution and causes of eutrophication in lakes in typical lake regions of China[J]. Journal of Ecology and Rural Environment, 2014,30(4):438-443.
|
| [2] |
黄文敏,邢伟,李敦海,等.微囊藻毒素-RR对烟草细胞活力及营养生理的影响[J]. 中国环境科学, 2007,27(3):382-386.
|
| [3] |
王伟琴,金永堂,吴斌,等.水源水中微囊藻毒素的遗传毒性与健康风险评价[J]. 中国环境科学, 2010,30(4):468-476.
|
| [4] |
Yin L Y, Huang J Q, Huang W M, et al. Microcystin-RR-induced accumulation of reactive oxygen species and alteration of antioxidant systems in tobacco BY-2cells[J]. Toxicon, 2005, 46(5):507-512.
|
| [5] |
徐慧敏,闫海,马松,等.鞘氨醇单胞菌USTB-05对微囊藻毒素的生物降解[J]. 中国环境科学, 2014,34(5):1316-1321.
|
| [6] |
张睿,王广军,李志斐,等.枯草芽孢杆菌对铜绿微囊藻抑制效果的研究[J]. 中国环境科学, 2015,35(6):1814-1821.
|
| [7] |
杨翠云,李敦海,刘永定.微囊藻毒素对典型微生物生长及生理生化特性的影响[J]. 水生生物学报, 2008,32(6):818-823.
|
| [8] |
杨翠云,夏传海,周世伟.微囊藻毒素-RR对大肠杆菌和枯草杆菌的渗透效应[J]. 科学通报, 2010,55(11):1002-1006.
|
| [9] |
尹黎燕,黄家权,沈强,等.烟草悬浮细胞抗氧化系统对微囊藻毒素-RR的响应[J]. 中国环境科学, 2005,25(5):576-580.
|
| [10] |
鲍忠祥.滇池浮游植物和微囊藻毒素的时空分布及细菌去除毒素研究[D]. 昆明,云南大学, 2012.
|
| [11] |
袁媛,吴涓,李玉成,等.活性炭纤维固定化菌对微囊藻毒素MC-LR的去除研究[J]. 中国环境科学, 2014,34(2):403-409.
|
| [12] |
Ding W X, Shen H M, Ong C N. Calpain activation after mitochondrial permeability transition in microcystin-induced cell death in rat hepatocytes[J]. Biochemical and Biophysical Research Communications, 2002,291(2):321-331.
|
| [13] |
肖巧巧,尹华,叶锦韶,等.一株微囊藻毒素-LR降解菌的降解特性[J]. 环境化学, 2014,33(9):1-7.
|
| [14] |
邓庭进,叶锦韶,彭辉,等.微囊藻毒素-LR对恶臭假单胞菌细胞活性和表面特性的影响[J]. 环境科学, 2015,36(1):252-258.
|
| [15] |
He Y Y, Häder D P. UV-B-induced formation of reactive oxygen species and oxidative damage of the cyanobacterium Anabaena sp.:protective effects of ascorbic acid and N-acetyl-L-cysteine[J]. Journal of Photochemistry and Photobiology B:Biology, 2002,66(2):115-124.
|
| [16] |
Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976,72(1):248-254.
|
| [17] |
Khan M M T, Pyle B H, Camper A K. Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry[J]. Applied and environmental microbiology, 2010,76(15):5088-5096.
|
| [18] |
Rurián-Henares J A, Morales F J. Antimicrobial activity of melanoidins against Escherichia coli is mediated by a membranedamage mechanism[J]. Journal of Agricultural and Food Chemistry, 2008,56(7):2357-2362.
|
| [19] |
Schalk I J, Hannauer M, Braud A. New roles for bacterial siderophores in metal transport and tolerance[J]. Environmental Microbiology, 2011,13(11):2844-2854.
|
| [20] |
王燕,陈永刚,葛郑增,等.TBT对大鼠肝脏ROS、抗氧化酶和解毒系统酶的影响[J]. 中国环境科学, 2005,25(4):428-431.
|
| [21] |
Codreanu S G, Liebler D C. Novel approaches to identify protein adducts produced by lipid peroxidation[J]. Free Radical Research, 2015(0):1-7.
|
| [22] |
Xiong Q, Xie P, Li H, et al. Acute effects of microcystins exposure on the transcription of antioxidant enzyme genes in three organs (liver, kidney, and testis) of male Wistar rats[J]. Journal of Biochemical and Molecular Toxicology, 2010,24(6):361-367.
|
| [23] |
?egura B, Štraser A, Filipi? M. Genotoxicity and potential carcinogenicity of cyanobacterial toxins-a review[J]. Mutation Research/Reviews in Mutation Research, 2011,727(1):16-41.
|
| [24] |
Pinho G L L, da Rosa C M, Maciel F E, et al. Antioxidant responses and oxidative stress after microcystin exposure in the hepatopancreas of an estuarine crab species[J]. Ecotoxicology and Environmental Safety, 2005,61(3):353-360.
|
| [25] |
Pflugmacher S, Wiegand C, Beattie K A, et al. Uptake, effects, and metabolism of cyanobacterial toxins in the emergent reed plant Phragmites australis (cav.) trin. ex steud[J]. Environmental Toxicology and Chemistry, 2001,20(4):846-852.
|
| [26] |
Wang Y, Hammes F, De Roy K, et al. Past, present and future applications of flow cytometry in aquatic microbiology[J]. Trends in biotechnology, 2010,28(8):416-424.
|
|
|
|
