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| The characteristics of extracellular polymeric substances and intracellular polymeric substances in activated sludge fed with tetracycline |
| ZHANG Jun-ke1,2,3, ZENG Ping2,3,4, SONG Yong-hui2,3, Yu Hui-bin2,3, GUAN dong-ming1, LIU Rui-xia1,2 |
1. School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China;
2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
3. Department of Urban Water Environmental Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
4. State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC), Tsinghua University, 100084 Beijing, China |
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Abstract Extracellular polymeric substances (EPS) and intracellular polymeric substances (IPS) of activated sludge feeding with tetracycline were divided into four parts, soluble extracellular polymeric substances (slime), loosely bound EPS (LB), tightly bound EPS (TB), intracellular polymeric substances (IPS) after extractionby centrifuge and ultrasonic process. Protein and polysaccharide were detected with BCA and anthrone-sulfuric acid method. At the same time, 3Dexcitation-emission spectral scan (EEM) and synchronous fluorescence spectrum scan (SFS) were applied to quantitatively and qualitatively analyze DOM in EPS and IPS. The results showed that the content of protein and polysaccharide in EPS was higher than that in IPS, and the content of protein was higher than that of polysaccharide. In slime and IPS, the content of protein and polysaccharide was higher than that in LB and TB. Tryptophan-like with low and high excitation wavelength was the main component of EPS and IPS. The content of microbial byproducts and aromatic proteins increased gradually in slime, LB, TB and IPS, while the refractory organic humic-like decreased. Influent tetracycline concentration has good correlation with DOM of EPS and IPS from activated sludge. And the DOM detected by EEM had good correlation with protein detected by chemical test.
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Received: 28 June 2015
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