基于流式细胞特性的LNA和HNA细菌相关性研究

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Abstract

In the present study, bacterial concentration and FCM characteristics of LNA and HNA were analyzed in seven different terrestrial environments. Results showed clear separation between LNA and HNA bacteria in both aquatic and soil environments. The LNA abundance in soil (10⁷~10⁸cells/g) was higher than that of freshwater (10⁵~10⁶cells/mL) while the proportion of LNA in soil (29.80%~33.94%) was lower than that of freshwater (42.25%~65.92%), but not underground water (21.60%). Principal component analysis (PCA) indicated that the flow cytometric characteristics of LNA and HNA had distinct differences between freshwater and soil. Correlation analysis further revealed that both green fluorescence (FL1) and side scatter (SSC) signals between LNA and HNA bacteria had a significant correlation (FL1:R²=0.711, P<0.01; SSC:R²=0.762, P<0.01), i.e. co-variation between LNA and HNA. The variance of SSC was higher than that of FL1 in different ecosystems. The results demonstrated that LNA and HNA bacteria were neither physiologically related nor completely independent communities, but rather have a close co-variation.

Key words： terrestrial ecosystems flow cytometry (FCM) low nucleic acid content (LNA) bacteria high nucleic acid content (HNA) bacteria DNA fluorescence staining

Received: 11 June 2015

PACS:

X172

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	LIU Jie
	SONG Yu-hao
	MA Rui
	WANG Ying-ying

Cite this article:

LIU Jie,SONG Yu-hao,MA Rui等. Correlation of LNA and HNA bacteria based on flow cytometric characteristics[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(3): 865-874.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I3/865

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