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| Isolation and identification of a heterotrophic nitrification-aerobic denitrification bacterium and its denitrification ability |
| ZOU Yan-yan1,2, ZHANG Yu1, LI Ming-zhi1, MEI Rong-wu1, WEI Yan-fei1, DING Lin-xian2 |
1. Project Research Center, Environmental Science Research and Design Institute of Zhejiang Province, Hangzhou 310007, China;
2. College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China |
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Abstract A bacterium ZB612 with heterotrophic nitrification-aerobic denitrification was isolated from soil sample around leachate regulating pool of Hangzhou Tianziling waste landfill. The strain ZB612 was preliminary identified as Rhizobium sp. by morphology and 16S rDNA homology analysis. We subsequently studied its denitrification ability, the results showed that the ammonia nitrogen removal efficiency reached 90% in the heterotrophic nitrification medium with initial ammonia nitrogen concentration of 100mg/L. Meanwhile, the obvious accumulation of nitrate-N and nitrite-N did not appeared, which is the characteristics of simultaneous nitrification and denitrification (SND). In nitrates denitrification system,the nitrate nitrogen removal efficiency could reach 60%. In addition, we investigated the effect of four factors (including temperature, pH, carbon nitrogen ratio and carbon source) on denitrification efficiency of the strain ZB612, respectively. The optimum denitrification conditions were found to be as follows: temperature of 30℃, the initial pH of 7, C/N ratio of 8, where the optimal carbon resource was glucose.
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Received: 07 August 2015
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