甲醛经口染毒致小鼠脏器损伤和炎症反应的研究

Abstract
Figure/Table
References (18)
Related Citation (15)

Download: PDF (509 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

To explore the effects on mice through administration of formaldehyde (FA) by gavage with or without ovalbumin (OVA), which lead to the inflammation in body, the male Balb/c mice were randomly divided into 7groups: the control group (distilled water group), OVA group, 2mg/(kg·d) FA (formaldehyde) group, 20mg/(kg·d) FA group, 200mg/(kg·d) FA group, 200mg/(kg·d) FA+OVA group and 200mg/(kg·d)FA+OVA+ MT (melatonin) group. Mice were gavaged with distilled water and different concentrations of FA solution respectively for 21 days. The mice in OVA group, 200mg/(kg·d) FA+OVA group and 200mg/(kg·d) FA+OVA+MT group were sensitized with OVA in day 6, 13, 20 by intraperitoneal injection, and the mice in 200mg/(kg·d) FA+OVA+MT group were also challenged with 1mg/mL MT by gavage for 21 days. The contents of ROS, MDA and GSH in liver, kidney and lung tissue homogenate were determined. IL-4and IFN-g concentrations in liver were measured by ELISA kits. The results showed that compared with control group, the livers ROS of mice in 200mg/(kg·d) FA group reached a significant level (P<0.05), and MDA increased significantly (P<0.05); GSH content in kidneys decreased significantly (P<0.05). IL-4 content in livers of mice in 200mg/(kg·d) FA+OVA group significantly increased (P<0.01). In 200mg/(kg·d) FA+OVA group, the administration of 10mg/(kg·d) MT can reduce ROS in the livers significantly (P<0.05). In a word, the gavage of 200mg/(kg·d) FA would induce oxidative damage and inflammation in mice. ROS, MDA in 200mg/(kg·d) FA group reached a significant level (P<0.05), on the contrary, GSH content decreased significantly (P<0.05). In 200mg/(kg·d) FA+OVA group, IL-4 content in livers of mice significantly increased (P<0.01), while IFN-g content had no significant change (P>0.05).

Key words： formaldehyde ovalbumin mice oxidative stress inflammation

Received: 19 June 2015

PACS:

X503.22

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	ZHAO Yun
	YOU Hui-hui
	SHEN Shi-Ping
	GUO Qing
	MEI Yu-fei
	LI Jin-quan
	ZHU Yu-qing
	LI Xiao-xiao
	DING Shu-mao
	YANG Xu

Cite this article:

ZHAO Yun,YOU Hui-hui,SHEN Shi-Ping等. Organ injure and inflammatory response of mice induced by oral exposure of formaldehyde[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(3): 935-942.

URL:

http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I3/935

[1]	Tang X, Bai Y, Duong A, et al. Formaldehyde in China: production, consumption, exposure levels, and health effects[J]. Environment International, 2009,36(8):1210-1224.
[2]	Sul D, Kim H, Oh E, et al. Gene expression profiling in lung tissues from rats exposed to formaldehyde[J]. Archive Für Toxikologie, 2007,81(8):589-597.
[3]	李慧,张玉超,柯玉洁,等.甲醛对原癌基因c-myc, MDM2及抑癌基因p53的影响[J]. 中国环境科学, 2013,33(8):1483-1486.
[4]	柯玉洁,秦晓丹,李兰,等.甲醛对小鼠骨髓组织的毒性作用[J]. 中国环境科学, 2012,32(6):1129-1133.
[5]	WHO. 2002. Concise International Chemical Assessment Document 40: Formaldehyde[R]. Geneva: World Health Organization. 12-16.
[6]	Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?[J]. British Journal of Pharmacology, 2004,142(2):231-255.
[7]	段丽菊,朱燕,胡青莲,等.甲醛吸入致小鼠蛋白质氧化损伤作用的研究[J]. 环境科学学报, 2005,25(6):851-854.
[8]	Riedel F, Hasenauer E, Barth P J, et al. Formaldehyde exposure enhances inhalative allergic sensitization in the guinea pig[J]. Allergy, 1996,51(2):94-99.
[9]	Tarkowski M, Gorski P. Increased IgE Antiovalbumin Level in Mice Exposed to Formaldehyde[J]. International Archives of Allergy & Immunology, 1995,106(4):422-424.
[10]	Kumar R K, Herbert C, Foster P S. The "classical" ovalbumin challenge model of asthma in mice[J]. Current Drug Targets, 2008,9(6):485-494(10).
[11]	Qiao Y, Li B, Yang G. Irritant and adjuvant effects of gaseous formaldehyde on the ovalbumin-induced hyperresponsiveness and inflammation in a rat model.[J]. Inhalation Toxicology, 2009,21(14):1200-1207.
[12]	Liu D, Zheng Y, Li B, et al. Adjuvant effects of gaseous formaldehyde on the hyper-responsiveness and inflammation in a mouse asthma model immunized by ovalbumin.[J]. Journal of Immunotoxicology, 2011, volume 8(4):305-314(10).
[13]	杨剑婷,吴彩娥,李莹莹,等.白果蛋白过敏动物模型的试验研究[J]. 中国农业科学, 2010,43(17):3616-3623.
[14]	武阳,常青,杨旭.不同浓度甲醛致大鼠肝细胞DNA氧化损伤作用[J]. 环境科学学报, 2009,29(11):2415-2419.
[15]	代国杰,朱建津,高琳琳,等.褪黑素对小鼠抗氧化作用的影响[J]. 食品工业科技, 2010,(3):347-350.
[16]	刘云儒,许琼军,马亚萍,等.甲醛灌胃染毒对小鼠肝脏超氧化物歧化酶和丙二醛的影[J]. 中华劳动卫生职业病杂志, 2014, 32(5):376-377.
[17]	Jung W W. Formaldehyde exposure induces airway inflammation by increasing eosinophil infiltrations through the regulation of reactive oxygen species production.[J]. Environmental Toxicology & Pharmacology, 2007,24(2):174-182.
[18]	Galano A, Tan D X, Reiter R J. Melatonin as a natural ally against oxidative stress: a physicochemical examination.[J]. Journal of Pineal Research, 2011,51(1):1-16.