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| Reactivation performance of nitrosation granular sludge after long-term storage and microbial community variation |
| QIAN Fei-yue1,2,3, WANG Yan1, WANG Jian-fang1,2,3, WANG Shu-yong1, SHEN Yao-liang1,2,3 |
1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;
2. Jiangsu Key Laboratory of Environmental Science and Engineering, Suzhou 215009, China;
3. Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215009, China |
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Abstract The reactivation performance of nitrosation granular sludge(NGS), which had been stored at 24~29℃ for a long-term of 1a, was investigated in a sequencing batch reactor(SBR) fed with inorganic synthetic wastewater, via the gradually increasing of the influent ammonia nitrogen loading from 0.32 to 0.64kg/(m3·d) and initial concentration of free ammonia from 3.7 to 7.2mg/L. High throughput sequencing technology of Miseq was also employed to analyze the variation of microbial community before and after the reactivation. Results showed that the stored NGS could be reactivated in a short period of 8days, since the removal effciency of ammonia nitrogen and the accumulation ratio of nitrite nitrogen were higher than 95% and 80%, respectively. Meanwhile, both sludge particle size and extracellular polymer substances(EPS) contents decreased significantly. After 20days reactivation, the specific ammonia nitrogen removal rate of 24.6mg/(gVSS·h) and the specific nitrite accumulation rate of 23.8mg/(gVSS·h) were observed, while average particle size of NGS remained at 0.5mm. During this period, the microbial diversity of NGS decreased obviously, ascribed to the washing out of most of anaerobic and heterotrophic bacteria. The relative abundance of ammonia-oxidizing bacteria, such as Nitrosomonas ect., increased from 1% to 58%, and the growth of nitrite-oxidizing bacteria was inhibited selectively. It indicated that the NGS after a long-term storage could be inoculated in SBR to achieve its rapid start-up.
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Received: 23 September 2015
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