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| Response of methanogens on temperature stress in an UASB reactor |
| ZHANG Li-guo1, BAN Qiao-ying1, LI Jian-zheng2 |
1. College of Environment and Resource, Shanxi University, Taiyuan 030006, China;
2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China |
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Abstract The effects of temperature stress on different nutrition type methanogens in an upflowed anaerobic sludge blanket(UASB) reactor was investigated by batch culture. The results showed that the dominant hydrogenotrophic and acetotrophic methanogens in this reactor were Methanospirillum and Methanosaeta, respectively. Under 35℃ condition, the accumulative methane production from hydrogenotrophic and acetotrophic methanogens were 24.7 and 11.7mL, separately. And the maximum methane production rate(Rmax) were 0.74 and 0.18mL/h, respectively. Temperature decreases, from 35℃ to 30, 25, 20, 15℃, resulted in accumulative methane productions from hydrogenotrophic methanogens were decreased by 14.2%, 34.0%, 47.0%, 57.5%, respectively. While those from acetotrophic methanogens were reduced by 5.1%, 23.9%, 45.3%, 95.7%, separately. These results indicated that hydrogenotrophic methanogens are more sensitive to temperature tress at 20~30℃, while acetotrophic methanogens are more sensitive at ≤15℃.
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Received: 18 September 2015
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