|
|
|
| Bacterial community structures of aerobic denitrification bacteria nitrogen removal process in source water experiment by using Miseq high-throughput sequencing technique. |
| ZHOU Shi-lei, HUANG Ting-lin, ZHANG Chun-hua, BAI Shi-yuan, HE Xiu-xiu |
| Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China |
|
|
|
|
Abstract Miseq high-throughput sequencing technique was used to investage the bacterial community structure of nitrogen removal process in source water experiment systems added aerobic denitrification bacteria. Bioinformatics analysis were carried on the for the samples of bacteria and control systems. Then, the fundamental analysis, Optimized sequence statistics, OTU distribution, and Taxonomic analysis; the Advanced analysis, the microbial community, PCA, Rank-Abundance, Hcluster, Specaccum, and Venn of OTU distribution. As a result, the nitrogen of bacteria system was removed obviously, and Hierarchical cluatering and PCA showed that the microbial community structure of bacteria and control systems has changed, and the Protebacterice and Bacteroidetes were the main phylum in experiment systems. Meanwhile, the N-functional microbial of bacteria system had an increase process, obviously, which was consistent with the changes of aerobic denitrification bacteria. From all the results, the Miseq high-throughput sequencing technique was an effective tool to explore the changes of bacterial community structure of nitrogen removal process, which could supply a reference to study the changes of community structure of remediation the source water in situ.
|
|
Received: 15 September 2015
|
|
|
|
|
|
| [1] |
Robertson L A, Kuenen J G. Thiosphaera pantotropha gen. nov. sp. nov., a facultatively anaerobic, facultatively autotrophic sulphur bacterium[J]. Journal of General Microbiology, 1983, 129(9):2847-2855.
|
| [2] |
Gao H, Schreiber F, Collins G, et al. Aerobic denitrification in permeable Wadden Sea sediments[J]. The ISME journal, 2010, 4(3):417-426.
|
| [3] |
Zhang J B, Wu P X, Hao B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001[J]. Bioresource Technology, 2011,102(21):9866-9869.
|
| [4] |
Yang X P, Wang S M, Zhang D W, et al. Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Bacillus subtilis A1[J]. Bioresource Technology, 2011,102(2):854-862.
|
| [5] |
Yu A, Li Y, Yu J. Denitrification of a newly isolated Bacillus strain W2and its application in aquaculture[J]. Journal of Microbiology, 2004,25(3):77-81.
|
| [6] |
Zhao B, An Q, He Y L, et al. N2O and N2production during heterotrophic nitrification by Alcaligenes faecalis strain NR[J]. Bioresource Technology, 2012,116(0):379-385.
|
| [7] |
Joo H S, Hirai M, Shoda M. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4[J]. Journal of Bioscience and Bioengineering, 2005,100(2):184-191.
|
| [8] |
Cleenwerck I, De Wachter M, Hoste B, et al. Aquaspirillum dispar Hylemon et al. 1973 and Microvirgula aerodenitrificans Patureau et al. 1998 are subjective synonyms[J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53:(1457-1459).
|
| [9] |
Patureau D, Helloin E, Rustrian E, et al. Combined phosphate and nitrogen removal in a sequencing batch reactor using the aerobic denitrifier, Microvirgula aerodenitrificans[J]. Water Research, 2001,35(1):189-197.
|
| [10] |
Chen Q, Ni J R. Heterotrophic nitrification-aerobic denitrification by novel isolated bacteria[J]. J. Ind. Microbiol. Biotechnol., 2011, 38(9):1305-1310.
|
| [11] |
Patureau D, Bernet N, Moletta R. Combined nitrification and denitrification in a single aerated reactor using the aerobic denitrifier Commonas sp. strain SGLY2[J]. Water Research, 1997, 31(6):1363-1370.
|
| [12] |
张培玉,曲洋,于德爽,等.菌株qy37的异养硝化/好氧反硝化机制比较及氨氮加速降解特性研究[J]. 环境科学, 2010,31(8):1819-1826.
|
| [13] |
于爱茸,李尤,俞吉安.一株耐氧反硝化细菌的筛选及脱氮特性研究[J]. 微生物学杂志, 2005,25(3):77-81.
|
| [14] |
Joo H-S, Hirai M, Shoda M. Piggery wastewater treatment using Alcaligenes faecalis strain No. 4with heterotrophic nitrification and aerobic denitrification[J]. Water Research, 2006,40(16):3029-3036.
|
| [15] |
Bouchez T, Patureau D, Delgen S J, et al. Successful bacterial incorporation into activated sludge flocs using alginate[J]. Bioresource Technology, 2009,100(2):1031-1032.
|
| [16] |
Wang P, Li X, Xiang M, et al. Characterization of efficient aerobic denitrifiers isolated from two different sequencing batch reactors by 16S-rRNA analysis[J]. Journal of Bioscience and Bioengineering, 2007,103(6):563-567.
|
| [17] |
Kuznetsov S, Dubinina G, Lapteva N. Biology of oligotrophic bacteria[J]. Annual Reviews in Microbiology, 1979,33(1):377-387.
|
| [18] |
Wainaright M, Barakah F, Al-Turk I, et al. Oligotrophic micro-organisms in industry, medicine and the environment[J]. Science Progress, 1990,75(298Pt 3-4):313-322.
|
| [19] |
Button D, Schut F, Quang P, et al. Viability and isolation of marine bacteria by dilution culture:theory, procedures, and initial results[J]. Applied and Environmental Microbiology, 1993,59(3):881-891.
|
| [20] |
黄廷林,周娜,张海涵,等.3株贫营养好氧反硝化细菌的分离鉴定及反硝化特性[J]. 环境工程学报, 2014,8(12):5507-5513.
|
| [21] |
黄廷林,魏巍,王春燕,等.扬水曝气-贫营养生物膜组合技术净化微污染原水中试研究[J]. 重庆大学学报, 2012,(1):125-131,146.
|
| [22] |
魏巍,黄廷林,苏俊峰,等.1株贫营养好氧反硝化菌的分离鉴定及其脱氮特性[J]. 生态环境学报, 2010,19(9):2166-2171.
|
| [23] |
黄廷林,苏俊峰,李倩.好氧反硝化菌株的筛选培养及其反硝化性能研究[J]. 西安建筑科技大学学报:自然科学版, 2009,(5):704-707.
|
| [24] |
白玉涛,周玉,赵吉.内蒙古高原干涸湖泊反硝化及甲烷氧化细菌的群落分析[J]. 中国环境科学, 2012,32(7):1293-1301.
|
| [25] |
党岩,张瑞,叶杰旭,等.EGSB处理垃圾焚烧渗沥液及其微生物群落变化[J]. 中国环境科学, 2013,33(6):999-1004.
|
| [26] |
郭建宁,陈磊,张锡辉,等.臭氧/陶瓷膜对生物活性炭工艺性能和微生物群落结构影响[J]. 中国环境科学, 2014,34(3):697-704.
|
| [27] |
郑林雪,李军,胡家玮,等.同步硝化反硝化系统中反硝化细菌多样性研究[J]. 中国环境科学, 2015,35(1):116-21.
|
| [28] |
赵文莉,郝瑞霞,王润众,等.复合碳源填料反硝化脱氮及微生物群落特性[J]. 中国环境科学, 2015,35(10):3003-3009.
|
| [29] |
Huang T L, Zhou S L, Zhang H H, et al. Nitrogen removal from micro-polluted reservoir water by indigenous aerobic denitrifiers[J]. International Journal of Molecular Sciences, 2015,16(4):8008-8026.
|
| [30] |
Wang Y, Sheng H F, He Y, et al. Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags[J]. Applied and environmental microbiology, 2012,78(23):8264-8271.
|
| [31] |
Oberauner L, Zachow C, Lackner S, et al. The ignored diversity:complex bacterial communities in intensive care units revealed by 16S pyrosequencing[J]. Scientific Reports, 2013,3:1413.
|
| [32] |
Fouts D E, Szpakowski S, Purushe J, et al. Next generation sequencing to define prokaryotic and fungal diversity in the bovine rumen[J]. PLOS One, 2012,7(11):e48289.
|
| [33] |
Jami E, Israel A, Kotser A, et al. Exploring the bovine rumen bacterial community from birth to adulthood[J]. The ISME journal, 2013,7(6):1069-1079.
|
| [34] |
国家环境保护总局.《水和废水监测分析方法》.第4版[M]. 北京:中国环境科学出版社, 2002.
|
| [35] |
Huang T L, Wei W, Su J F, et al. Denitrification performance and microbial community structure of a combined WLA-OBCO system[J]. PlOS One, 2012,7(11):e48339.
|
| [36] |
Bates S T, Clemente J C, Flores G E, et al. Global biogeography of highly diverse protistan communities in soil[J]. The ISME journal, 2013,7(3):652-659.
|
| [37] |
Yan Q, Bi Y, Deng Y, et al. Impacts of the Three Gorges Dam on microbial structure and potential function[J]. Scientific Reports, 2015,5:8605.
|
| [38] |
Yang X, Xie P, Ma Z, et al. Decrease of NH4+-N by bacterioplankton accelerated the removal of cyanobacterial blooms in aerated aquatic ecosystem[J]. Journal of Environmental Sciences, 2013,25(11):2223-2228.
|
| [39] |
Leibold M A, Holyoak M, Mouquet N, et al. The metacommunity concept:a framework for mult-scale community ecology[J]. Ecology Letters, 2004,7(7):601-613.
|
| [40] |
Brendan Logue J, Lindström E S. Biogeography of bacterioplankton in inland waters[J]. Freshwater Reviews, 2008,1(1):99-114.
|
|
|
|
