乳酸对铜绿微囊藻的抑藻效应及机理

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Abstract

The inhibitory effect and possible inhibitory mechanisms of lactic acid on M. aeruginosa were studied. The results showed that lactic acid had a strong inhibiting effect on the growth of M. aeruginosa, 72h, except the inhibition rate of experimental group in lowest concentration was 60%, the rest inhibition rate of experimental group were reached more than 80%; under the stress of lactic acid, the contents of nucleic acid and protein, the electrical conductivity(EC) in M. aeruginosa supernatant all increased, the contents of malondialdehyde(MDA) and oxygen free radical(O₂^·) of M. aeruginosa cells enhanced, the activity of superoxide dismutase(SOD) decreased, and transmission electron microscope photographs showed that ultrastructure of M. aeruginosa cells had changed obviously. All the results indicated that the possible inhibiting mechanisms were that lactic acid changed the permeability of M. aeruginosa membrane and the cell structure, decreased its antioxidant capacity, and caused the crack and death of the cell in the end.

Key words： lactic acid Microcystis aeruginosa inhibitory effect inhibitory mechanism

Received: 10 September 2015

PACS:

X503

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	Articles by authors
	PENG Gui-ying
	CHEN Yong-ling
	HAN Yu-zhen
	ZHANG Ting-ting

Cite this article:

PENG Gui-ying,CHEN Yong-ling,HAN Yu-zhen等. The inhibitory effect of lactic acid on Microcystis aeruginosa and its mechanisms[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(4): 1167-1172.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I4/1167

[1]	João G F, Jesper H A, Angel B, et al. Overview of eutrophication indicators to assess environmental status within the European Marine Strategy Framework Directive[J]. Estuarine, Coastal and Shelf Science, 2011,93:117-131.
[2]	孙雪梅,刘碧云,鲁志营,等.焦性没食子酸对铜绿微囊藻的氧化胁迫效应研究[J]. 中国环境科学, 2013,33(10):1835-1841.
[3]	Zhang T T, He M., Nie L W. Allelopathic effect of surbmerbed macrophyte Chara vulgaris on toxic Microcystis aeruginosa[J]. Allelopathy Journal, 2009,23(2):391-402.
[4]	张庭廷,郑春艳,何梅,等.脂肪酸类物质的抑藻效应及其构效关系[J]. 中国环境科学, 2009,29(3):274-279.
[5]	王红强,张丽萍,张胜花,等.伊乐藻中有机酸的GC-MS分析及其抑藻作用研究[J]. 环境科学与技术, 2011,34(7):23-26.
[6]	Xian Q M, Chen H D, Qu L J, et al. Allelopathic potential of aqueous extracts of submerged macrophytes against algal growth[J]. Allelopathy Journal, 2005,15(1):95-104.
[7]	Sun X X, Choi J K, Kim E K. A preliminary study on the mechanism of harmful algal bloom mitigation by use of sophorolipid treatment[J]. Journal of Experimental Marine Biology and Ecology, 2004,304(1):35-49.
[8]	Heath R L, Parker L. Photoperitation in isolated chloroplasts kinetics and stoichiometry of fatty acid peroxidation[J]. Arch Biophy, 1968,75(2):189-198.
[9]	Stewert R C, Bewley J D. Lipid peroxidation associated with accelerated again of soybean axes[J]. Plant Physiol., 1980,65(3):245-248.
[10]	萧华山,何文锦,傅文庆,等.一种用分光光度计检测氧自由基的新方法[J]. 生物化学与生物物理进展, 1999,26(2):180-182.
[11]	Zhang T T, Hu W, Zhang D. Allelopathic effect of Typha angustifolia L. on phytoplankton[J]. Adv. Mater. Res., 2012:3724-3728.
[12]	吴红淼,王晓鹏,王磊,等.鱼腥草多糖的抑菌作用[J]. 中国野生植物资源, 2012,5(31):24-26.
[13]	刘光涛,周长芳,孙利芳.凤眼莲化感物质对铜绿微囊藻、斜生栅藻生长及细胞数相对比例的影响[J]. 环境科学学报, 2011, 31(10):2303-2311.
[14]	张庭廷,郑春艳,何梅,等.亚油酸对铜绿微囊藻的抑制机理[J]. 中国环境科学, 2009,29(4):419-424.
[15]	许文武,胡威,章典,等.焦性没食子酸对3种不同水生生物的毒性作用[J]. 卫生研究, 2011,40(2):172-174.
[16]	李峰民,胡洪营,种云霄,等.2-甲基乙酰乙酸乙酯对藻细胞膜和亚显微结构的影响[J]. 环境科学, 2007,28(7):1534-1539.
[17]	李寿田,周健民,王火焰,等.植物化感作用机理的研究进展[J]. 农村生态环境, 2001,17(4):52-55.
[18]	Chang X X, Eigemann F, Hilt S. Do macrophytes support harmful cyanobacteria? Interactions with a green alga reverse the inhibiting effects of macrophyte allelochemicals on Microcystis aeruginosa[J]. Harmful Algae, 2012,19:76-84.
[19]	张彬,王书敏,刘小兵,等.植物化感抑藻物质研究[J]. 科技信息计术, 2011,37(4):115-121.
[20]	Li F M, Hu H Y. Isolation and characterization of a novel antialgal allelochemical from Phragmites communis[J]. Applied and Environmental Microbiology, 2005,71:6545-6553.
[21]	章典.复合化感物质抑藻效应及其对微囊藻毒素生物合成影响的研究[D]. 芜湖:安徽师范大学生命科学学院, 2012.
[22]	Macías F A, Galindo J L G, García-Díaz M D, et al. Allelopathic agents from aquatic ecosystems:potential biopesticides models[J]. Phytochemistry Reviews, 2008,7(1):155-178.