颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究

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Abstract

Different types of granule-based anaerobic biofilm (with and without carriers; conductive and non-conductive carriers) were adopted to alleviate the inhibition of propionic acid degradation under high H₂ partial pressure. And their performance was monitored and compared, at the beginning and end of biofilm cultivation. Moreover, microbial SEM images and community composition were investigated to analyze mechanisms involved in propionic acid degradation. The results showed that both conductive carbon-felt based biofilm and anaerobic granules were effective at promoting propionic acid degradation under high H₂ partial pressure, with maximum rate of 2.2 and 1.2mmol/h respectively. Propionic acid seemed to be degraded mainly via direct interspecies electron transfer (DIET) between acidogenic bacteria (Thermovirga, Levilinea, Syntrophomonas) and methanogens (Methanosaeta) for carbon-felt based biofilm. Nevertheless, degradation of propionic acid by anaerobic granules was possibly attributed to syntrophic operation of acidogenic bacteria (syntrophobacter) and methanogens (Methanolinea, Methanobacterium).

Key words： granule-based biofilm conductive carriers high H₂ partial pressure propionic acid degradation anaerobic granules

Received: 05 November 2015

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X705

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	Articles by authors
	XU Heng
	WANG Cui-Ping
	YAN Kun
	MENG Yao
	LIU Xiao-Ji
	WANG Kai-Jun

Cite this article:

XU Heng,WANG Cui-Ping,YAN Kun等. Granule-based anaerobic biofilm enhances propionic acid degradation under high H₂ partial pressure[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(5): 1435-1441.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I5/1435

[1]	徐恒,汪翠萍,王凯军.废水厌氧处理反应器功能拓展研究进展 [J]. 农业工程学报, 2014,30(18):238-248.
[2]	Xu H, Wang K, Holmes D E. Bioelectrochemical removal of carbon dioxide (CO2): An innovative method for biogas upgrading [J]. Bioresource technology, 2014,173:392-3928.
[3]	Ryckebosch E, Drouillon M, Vervaeren H. Techniques for transformation of biogas to biomethane [J]. Biomass and Bioenergy, 2011,35(5):1633-1645.
[4]	Xu H, Gong S, Sun Y, et al. High-rate hydrogenotrophic methanogenesis for biogas upgrading: the role of anaerobic granules [J]. Environmental Technology, 2014,36(4):529-537.
[5]	Luo G, Johansson S, Boe K, et al. Simultaneous hydrogen utilization and in situ biogas upgrading in an anaerobic reactor [J]. Biotechnology and bioengineering, 2012,109(4):1088-1094.
[6]	常风民,王启宝,Giwa S,等.城市污泥两段式催化热解制合成气研究 [J]. 中国环境科学, 2015,35(3):804-810.
[7]	褚春凤,孔海南,片岡直明.FISH法作为厌氧消化系统运行管理指标的可行性 [J]. 中国环境科学, 2003,23(2):192-195.
[8]	Muller N, Worm P, Schink B, et al. Syntrophic butyrate and propionate oxidation processes: from genomes to reaction mechanisms [J]. Environmental microbiology reports, 2010,2(4): 489-499.
[9]	Fukuzaki S, Nishio N, Shobayashi M, et al. Inhibition of the Fermentation of Propionate to Methane by Hydrogen, Acetate, and Propionate [J]. Applied and Environmental Microbiology, 1990,56(3):719-723.
[10]	孙寓姣,左剑恶,李建平,等.厌氧颗粒污泥中微生物种群变化的分子生物学解析 [J]. 中国环境科学, 2006,26(2):183-187.
[11]	高靖伟,赫婷婷,程翔,等.EGSB-MBR组合工艺处理糖蜜发酵废水效能研究 [J]. 中国环境科学, 2015,35(5):1416-1422.
[12]	杨平,方治华,石炎福.多孔聚合物载体与活性炭载体用于厌氧流化床处理有机废水的比较 [J]. 环境科学, 2001,31(1):45-48.
[13]	Macleod F A, Guiot S R, Costerton J W. Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor [J]. Applied and Environmental Microbiology, 1990,56(6):1598-1607.
[14]	Rotaru A-E, Shrestha P M, Liu F, et al. A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane [J]. Energy & Environmental Science, 2014,7(1):408-415.
[15]	Li H, Chang J, Liu P, et al. Direct interspecies electron transfer accelerates syntrophic oxidation of butyrate in paddy soil enrichments [J]. Environmental Microbiology, 2015,17(5):1533-1547.
[16]	徐恒,汪翠萍,王凯军.不同碳源对产甲烷生物阴极性能的影响 [J]. 高等学校化学学报, 2015,36(2):344-348.
[17]	Dahle H, Birkeland N-K. Thermovirga lienii gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, amino-acid-degrading bacterium isolated from a North Sea oil well [J]. International Journal of Systematic and Evolutionary Microbiology, 2006,56(7): 1539-1545.
[18]	Yamada T, Sekiguchi Y, Hanada S, et al. Anaerolinea thermolimosa sp. nov., Levilinea saccharolytica gen. nov., sp. nov. and Leptolinea tardivitalis gen. nov., sp. nov., novel filamentous anaerobes, and description of the new classes Anaerolineae classis nov. and Caldilineae classis nov. in the bacterial phylum Chloroflexi [J]. International Journal of Systematic and Evolutionary Microbiology, 2006,56(6):1331-1340.
[19]	Zhang C, Liu X, Dong X. Syntrophomonas erecta sp. nov., a novel anaerobe that syntrophically degrades short-chain fatty acids [J]. International Journal of Systematic and Evolutionary Microbiology, 2005,55(2):799-803.
[20]	Narihiro T, Terada T, Ohashi A, et al. Quantitative detection of previously characterized syntrophic bacteria in anaerobic wastewater treatment systems by sequence-specific rRNA cleavage method [J]. Water Research, 2012,46(7):2167-2175.
[21]	Imachi H, Sakai S, Sekiguchi Y, et al. Methanolinea tarda gen. nov., sp. nov., a methane-producing archaeon isolated from a methanogenic digester sludge [J]. International Journal of Systematic and Evolutionary Microbiology, 2008,58(1):294-301.
[22]	Van Eerten-Jansen M C A A, Veldhoen A B, Plugge C M, et al. Microbial Community Analysis of a Methane-Producing Biocathode in a Bioelectrochemical System [J]. Archaea, 2013, 2013:1-12.
[23]	Ferry J G, Smith P H, Wolfe R S. Methanospirillum, a New Genus of Methanogenic Bacteria, and Characterization of Methanospirillum hungatii sp.nov [J]. International Journal of Systematic and Evolutionary Microbiology, 1974,24(4):465-469.
[24]	Morita M, Malvankar N S, Franks A E, et al. Potential for direct interspecies electron transfer in methanogenic wastewater digester aggregates [J]. M. Bio., 2011,2(4):e00159-11.