北运河沉积物中主要脱氮功能微生物的群落特征

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Abstract

Denitrification and anaerobic ammonia oxidation are two main processes for nitrogen removal in nitrogen cycle. The seasonal variation of community diversity and abundance, phylogenetic composition of nirS-type denitrifiers and Anammox (anaerobic ammonia oxidation) bacteria of sediments in Beiyun River were compared based on PCR (polymerase chain reaction), T-RFLP (terminal restriction fragment length polymorphism), clone and sequencing. The abundance of nirS-type denitrifiers increased from summer to winter while the abundance of Anammox bacteria decreased significantly. What's more, the abundance of nirS-type denitrifiers was significantly higher than Anammox in fall and winter. Community composition of the two microbial groups varied seasonally and the community diversity of nirS-type denitrifiers was much higher than Anammox bacteria. Concentrations of nitrogens and organic carbon in the sediments increased significantly from summer to winter. Environmental temperature was significantly correlated with the seasonal changes of abundance and community distribution of the two microbial groups in sediments. Correlation analysis revealed that total nitrogen had a great effect on the abundance of nirS-type denitrifiers, while C/N was significantly correlated with abundance of Anammox bacteria. NO_x^- and pH were also the main environmental factors determining the community distribution of nirS-type denitrifiers and Anammox bacteria in sediments. Phylogenetic analysis revealed that most of the denitrifying microbes belonged to species with relatively high pollution-resistance and efficiency of nitrogen removal. Phylogenetic diversity of nirS-type denitrifiers was much higher than that of Anammox bacteria. nirS-type denitrifiers were grouped into genera Pseudomonas and Halomonas, while Anammox was mainly bacteria belonged to Candidatus Brocadia.

Key words： Beiyun River sediment nirS-type denitrifiers Anammox seasonal change environmental factors phylogeny

Received: 08 October 2015

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X172

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	Articles by authors
	BAO Lin-lin
	WANG Xiao-yan
	CHEN Yong-juan
	ZHANG Ling-rong

Cite this article:

BAO Lin-lin,WANG Xiao-yan,CHEN Yong-juan等. Diversity, abundance and distribution of nirS-type denitrifiers and Anammox bacteria in sediments of Beiyun River[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(5): 1520-1529.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I5/1520

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