北京大气PM<sub>2.5</sub>对A549细胞炎性因子及DNA损伤的毒性

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Abstract

In order to investigate the toxicity effect of atmospheric PM_2.5 and its different fractions to human pulmonary epithelial cells A549 with respect to the relationship between PM_2.5 dosage and cell response, the water-soluble, fat-soluble, pure particle fractions of PM_2.5 (designated WSP_2.5, FSP_2.5 and PPP_2.5, respectively) prepared from original PM_2.5 samples (designated OP_2.5) and OP_2.5 were to treat A549 cells at different PM concentrations (10, 50, 100, 200, 400μg/mL, respectively). The MTS method was used to test the cell viability at 6h, 10h, 24h, 48h and 72h post-treatment, while ELISA and RT-QPCR were employed to detect the production of inflammatory cytokines IL-6 and TNF-α and the AP-sites counting was conducted to determine the level of intracellular DNA damage at 24h post-treatment. WSP_2.5 had very limited effect to inhibit cell growth and induce inflammatory damages and DNA base deletion. FSP_2.5, PPP_2.5 and OP_2.5 at high concentrations showed the inhibitory effect to cell growth across treatment; when cells were treated with low concentrations of FSP_2.5, PPP_2.5 or OP_2.5, the inhibition of cell growth occurred within a short period and then disappeared over time. FSP_2.5, PPP_2.5 andOP_2.5 treatment significantly induced the production of IL-6at both mRNA and protein levels, while WSP_2.5, PPP_2.5 andOP_2.5 treatment significantly induced the mRNA expression of TNF-α. FSP_2.5, PPP_2.5 and OP_2.5 treatment also induced the considerable DNA base deletion. In a word, not only the complex components adsorbed on the solid core granules of PM_2.5, but also the solid core granules themselves contributed to the cytotoxicity effects.

Key words： PM_2.5 fractions A549 cells cell viability inflammatory cytokines DNA damage

Received: 18 October 2015

PACS:

X503.1

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	Articles by authors
	JIAO Zhou-guang
	FU Xu-lei
	WEN Zhan-bo
	LI Jin-song
	LI Na
	ZHANG Ke
	WANG Jie
	HU Ling-fei

Cite this article:

JIAO Zhou-guang,FU Xu-lei,WEN Zhan-bo等. Toxicological study at inflammatory factors and DNA damages effects of Beijing atmospheric PM_2.5 and its different fractions to pulmonary epithelial cells A549 of human[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(5): 1579-1588.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I5/1579

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