光谱法研究胞外聚合物与四溴双酚A的相互作用

Abstract
Figure/Table
References (25)
Related Citation (15)

Download: PDF (651 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract

Three-dimensional fluorescence spectroscopy and infrared spectroscopy were used to investigatethe interaction between tetrabromobisphenol A (TBBA) and extracellular polymeric substance (EPS) from sludge. There were three particular fluorescence peaks in spectra of EPS, E_x/E_m=230/300nm (peak A), E_x/E_m=240/350nm (peak B) and E_x/E_m=270/370nm (peak C), respectively. The results of fluorescence titration revealed that the three fluorescence peaks of EPS could be quenched by TBBA. The binging constants (lg K) were calculated by the modified Stern-Volmer model and the Ryan-Weber model, ranged from 4.23 to 6.27. Infrared spectroscopy and synchronous fluorescence spectroscopy indicated the combination of EPS with TBBA has led to a change in EPS structure. The effect of environmental condition (i.e. pH, ions, and conductivity) on TBBA binding with EPS were evaluated. The results show that pH and ions affected binding affinity, but the effect ofconductivitywas minor.

Key words： EPS TBBA three-dimensional fluorescence spectrum binding

Received: 06 November 2015

PACS:

X131.2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	BAO Yi-jun
	YANG Cun-man
	LI Ying
	LU Guang-hua

Cite this article:

BAO Yi-jun,YANG Cun-man,LI Ying等. Spectroscopic methods study of the interaction of extracellular polymeric substance and tetrabromobisphenol A[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(6): 1773-1779.

URL:

http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I6/1773

[1]	彭浩,金军,王英,等.四溴双酚-A及其环境问题 [J]. 环境与健康杂志, 2006,23(6):571-573.
[2]	时国庆,李栋,卢晓珅,等.环境内分泌干扰物质的健康影响与作用机制 [J]. 环境化学, 2011,30:211-223.
[3]	Henley D V, Korach K S. Physiological effects and mechanisms of action of endocrine disrupting chemicals that alter estrogen signaling [J]. Hormones, 2010,9(3):191-205.
[4]	Gutierrze T, Biller D V, Shimmield T, et al. Metal binding properties of the EPS produced by Halomonas sp. TG39 and its potential in enhancing trace element bioavailability to eukaryotic phytoplankton [J]. Biometals, 2012,25(6):1185-1194.
[5]	张俊珂,曾萍,宋永会,等.受四环素影响的活性污泥胞内外聚合物特征 [J]. 中国环境科学, 2016,36(3):751-758.
[6]	简磊,李炳辉,郭训文,等.胞外聚合物对重金属离子的吸附 [J]. 广东化工, 2015,42(9):143-146.
[7]	Lindberg R H, Wennberg P, Johansson M I, et al. Screening of human antibiotic substances and determination of weekly mass flows in five sewage treatment plants in Sweden [J]. Environmental Science & Technology, 2005,39(10):3421-3429.
[8]	Yang S-F, Lin C-F, Lin A Y-C, et al. Sorption and biodegradation of sulfonamide antibiotics by activated sludge: experimental assessment using batch data obtained under aerobic conditions [J]. Water Research, 2011,45(11):3389-3397.
[9]	Sheng G-P, Zhang M-L, Yu H-Q. Characterization of adsorption properties of extracellular polymeric substances (EPS) extracted from sludge [J]. Colloids and Surfaces B: Biointerfaces, 2008, 62(1):83-90.
[10]	Xu J, Sheng G P, Ma Y, et al. Roles of extracellular polymeric substances (EPS) in the migration and removal of sulfamethazine in activated sludge system [J]. Water Research, 2013,47(14): 5298-5306.
[11]	郑蕾,田禹,孙德智. pH值对活性污泥胞外聚合物分子结构和表面特征影响研究 [J]. 环境科学, 2007,28(7):1507-1511.
[12]	Dubios M, Gilles K A, Hamilton J K, et al. Colorimetric Method for Determination of Sugars and Related Substances [J]. Anal. Chem., 1956,28(3):350-6.
[13]	Lowry O C, Rosebrough N. Protein measurement with the Folin phenol reagent [J]. Journal of Biologocal Chemistry, 1951,193(1): 265-275.
[14]	Fang F, Kanan S, Patterson H H, et al. A spectrofluorimetric study of the binding of carbofuran, carbaryl, and aldicarb with dissolved organic matter [J]. Analytica Chimica Acta, 1998,373(2):139-151.
[15]	冯启言,张彦,孟庆俊.煤矿区废水中溶解性有机质与铜的结合特性 [J]. 中国环境科学, 2013,33(8):1433-1441.
[16]	Wu F, Bai Y, Mu Y, et al. Fluorescence quenching of fulvic acids by fullerene in water [J]. Environmental Pollution, 2013,172: 100-107.
[17]	魏群山,罗专溪,陈强,等.天然水体溶解性有机物(DOM)分级组分对典型城市源污染的荧光响应 [J]. 环境科学研究, 2010,10:1229-1235.
[18]	Baker A, Inverarity R.Protein-like fluorescence intensity as a possible tool for determining river water quality [J]. Hydrological Processes, 2004,18(15):2927-2945.
[19]	傅平青,刘丛强,吴丰昌.三维荧光光谱研究溶解有机质与汞的相互作用 [J]. 环境科学, 2004,25(6):140-144.
[20]	安莹,王志伟,李彬,等.盐度冲击下MBR污泥SMP和EPS的三维荧光光谱解析 [J]. 中国环境科学, 2014,34(7):1754-1762.
[21]	Guibaud G, Tixier N, Bouju A, et al. Relation between extracellular polymers' composition and its ability to complex Cd, Cu and Pb [J]. Chemosphere, 2003,52(10):1701-1710.
[22]	Guibaud G, Comte S, Bordas F, et al. Comparison of the complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel [J]. Chemosphere, 2005,59(5):629-638.
[23]	Al-qadiri H M, Al-holy M A, Lin M, et al. Rapid detection and identification of Pseudomonas aeruginosa and Escherichia coli as pure and mixed cultures in bottled drinking water using Fourier transform infrared spectroscopy and multivariate analysis [J]. Journal of Agricultural and Food Chemistry, 2006,54(16):5749-5754.
[24]	Bi S, Yan L, Pang B, et al. Investigation of three flavonoids binding to bovine serum albumin using molecular fluorescence technique [J]. Journal of Luminescence, 2012,132(1):132-140.
[25]	梅毅,吴丰昌,王立英,等.运用3DEEMs及荧光偏振方法研究pH,离子强度及浓度效应对腐殖酸荧光光谱特性的影响 [J]. 地球化学, 2008,37(2):165-173.