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| Analysis of microbial community structure and functional bacteria in a biofilter with different sequencing technologies |
| CAI Yan-an1, LI Dong2, BI Xue-jun1, ZENG Hui-ping2, ZHANG Jie2,3 |
1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China;
2. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China;
3. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China |
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Abstract Effects of the technologies of clone library and high throughout sequencing for revealing microbial community structure and functional bacteria in a biofilter which simultaneously removes iron, manganese and ammonia were compared. 15057 sequences with 32 classifications in class level were obtained after the high throughout sequencing process, while there are 9classified groups in clone library. The former revealed more diversity of bacterial community structure. However, in the functional bacteria analysis process, some nitrifiers or iron and manganese bacteria which were detectable in one sequencing analysis process were not appeared in the other process. Comparing with using these two technologies individually in the analysing process, the combined using can compensate for each other and reveal the functional bacteria better.
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Received: 28 November 2015
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