Effect of pretreatment condition, H₂O and O₂ content on NO adsorption on Cu-ZSM-5molecular sieve was investigated in this experiment. The results showed that in the condition of 5% oxygen and none water Cu-ZSM-5 molecular sieve with the Si/Al ratio of 25had the best adsorption effect, whose breakthrough absorption amount was 0.8371mmol per gram. It had proved that water could be effectively removed by the non-thermal plasma(NTP) pretreatment on Cu-ZSM-5 molecular sieve using FT-IR method, and adsorption site of -OH retained. The water inhibited the adsorption capacity on Cu-ZSM-5 molecular sieve effectively, and breakthrough absorption amount was 0.09566mmol per gram. However, the adsorption capacity of mixed adsorbent, with the carbon molecular sieve(CMS)/La-Cu-ZSM-5 volume of nine, reached to 0.1889mmol per gram when the water content was 8%. While oxygen promoted the adsorption of nitric oxide, it inhibited the adsorption capacity on Cu-ZSM-5molecular sieve when the volume fraction of oxygen was 10%; TPD and FT-IR analysis showed adsorption species on Cu-ZSM-5molecular sieve was composed of NO₂, NO and -NO₃, when water content was 8%, the content of -NO₃ species was low.