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Effect of nutrient solution content of biofilm on algal growth and lipid accumulation |
XIONG Wei1,2, HUANG Yun1,2, FU Qian1,2, ZHONG Nian-bing1,2, ZHU Xun1,2, LIAO Qiang1,2 |
1. Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Ministry of Education, Chongqing University, Chongqing 400030, China;
2. Institute of Engineering Thermophysics, Chongqing University, Chongqing 400030, China |
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Abstract Agarose gel were coupled with mixed cellulose (CA-CN) membrane to build a photosynthetic microalgal biofilm reactor containing a two-layer source/substrate structure. The agarose gel layer was used to supply nutrient, while the CA-CN membrane layer was used as the framework for biofilm. The effects of nutrient contents on microalgal growth and lipid accumulation were investigated. As the agar powder content in culture increased from 0.125% to 8% (W/W), the nutrient contents decreased from 0.62 to 0.25g, the cell diameter decreased from 3.87 to 3.35μm, the thickness of microalgal biofilm decreased from 91.23 to 62.33μm, and the water content per unit biofilm thickness reduced by 40.49%. Consequently, the CO2 and light transfer resistance of internal biofilm decreased, whereas the areal density of microalgal biofilm was improved from 40.56 to 59.16g/m2 (45.86% increase), and the lipid production increased by 63.39%.
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Received: 15 January 2016
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