The dried biomass of dominate species of algae blooms, Microcystis, was used as a biosorbent to remove Sb(V) from effluents in this study. The biosorption characteristics of Sb (V) by Microcystis were investigated under various environmental parameters, biosorbent dosage, initial pH and contact time.The possible mechanisms were speculated based on the zeta potential and ATR-IR spectra analysis. Reaction equations between anionic Sb (V) and functional groups on the cell surface of Microcystis were inferred. The maximalbiosorption capacity was determined to be 5.84mg/g(dry weight) under biomass to solution ratio of 0.5g: 20mL, pH 2.0, equilibrium time 1h and room temperature. The biosorption isotherms were fitted well with Langmuir model (R²=0.993) and the biosorption kinetics process obeyed pseudo-secondorder rate kinetics equation (R²=0.994). The biosorption capacities of Sb (V) decreased with an increase of pH from 2.0to 9.0. The results of zeta potential and ATR-IR spectra analysis suggestthe involvement of amino, carboxyl and hydroxyl groups in Sb (V) biosorption. The protonated amine groups may bind anionic Sb (V) through electrostatic attraction. The protonated carboxyl groups and hydroxyl group might form an inner complex with Sb (V) through surface complexation.