基于454高通量测序的黄土高原不同乔木林土壤细菌群落特征

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Abstract

To determine the diversity of soil bacterial communities and its affecting factors with the method of high-throughput 454pyrosequencing technology, Soil samples were collected from four different arbor forests (Quercus liaotungensis, LDL; Biota orientalis, CB; Ptabulaeformis Carr; YS; Robinia pseucdoacacia, CH)), which represented the dominant communities for the forest vegetation ecosystem in the northwest of the Loess Plateau. The results showed that the structures of the microbial communities differed in terms of both the predominant phylum and the relative abundance of each phylum. At the phylum level, the dominant phyla were Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi and Planctomycetes. At the class level, the Actinobacteri, Alphaproteobacteria, Acidobacteria, Betaproteobacteria and Planctomycetacia were predominant. Compared with other arbor forests, the relative abundance of Acidobacteria and Bacteroidetes for LDL were the most abundant, while the relative abundance of Chloroflexi in YS and CB were more abundant than other vegetation types. Soil pH was significantly correlated with the relative abundance of Chloroflexi, and soil total phosphorus was significantly correlated with the relative abundance of Cyanobacteria, suggesting that soil total phosphorus was the main factor of affecting soil bacterial communities.

Key words： 454 high-throughput sequencing Ziwuling mountain different arbor forests soil bacteria environment factors

Received: 15 March 2016

PACS:

S153.2

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	Articles by authors
	LIU Yang
	ZENG Quan-chao
	HUANG Yi-mei

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LIU Yang,ZENG Quan-chao,HUANG Yi-mei. Soil microbial communities by 454prosequencing under different arbor forests on the Loess Plateau[J]. CHINA ENVIRONMENTAL SCIENCECE, 2016, 36(11): 3487-3494.

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http://manu36.magtech.com.cn/Jweb_zghjkx/EN/ OR http://manu36.magtech.com.cn/Jweb_zghjkx/EN/Y2016/V36/I11/3487

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